Morphometric | Abbreviation | Unit | Measurement |
---|---|---|---|
Fruit traits | |||
Cone diameter | CD | mm | Three measurements across the widest portion with a digital caliper |
Cone length | CL | mm | Measured using a digital caliper |
Cone weight | CW | g | 3-year-old cones were weighed with precision scale (accuracy of 0.01 g)a |
Seeds cone−1 | SN | No. | All seeds per cone were extracted and counted |
Empty seeds | ES | % | ES = (SN-PN) / SN |
Seed yield | SY | % | SY = ((SN × SW) / CW) × 100 |
Seed diameter | SD | mm | Three measurements across the widest portion with a digital caliper |
Seed length | SL | mm | Measured using a digital caliper |
Seed weight | SW | g | Weighed with precision scale (accuracy of 0.01 g)b |
Pine nuts cone−1 | PN | No. | All extracted seeds per cone were immersed and sinking seeds were counted |
Pine nut weight | PW | g | Five sound seeds and empty seeds were weighed from each cone. Calculated differences average five sound seeds weight and empty seeds weight per cone2. |
Pine nut yield | PY | % | PY = ((PN × PW) / CW) × 100 |
Seedling traits | |||
Number of cotyledons | NC | No. | Counted in 175 seedlings (7 seedlings × 5 replications × 5 populations) |
Height | SdL1 | cm | Measured by scale at the end of first vegetation period in 175 seedlings |
Height | SdL2 | cm | Measured by scale at the end of second vegetation period in 175 seedlings |
Root collar diameter | SdD1 | mm | Measured by digital caliper at the end of first vegetation period in 175 seedlings |
Root collar diameter | SdD2 | mm | Measured by digital caliper at the end of second vegetation period in 175 seedlings |
Lateral branches | LB1 | No. | Counted in 175 seedlings at the end of first vegetation period |
Lateral branches | LB2 | No. | Counted in 175 seedlings at the end of second vegetation period |
Bud set | BS | % | Observed in 175 seedlings at the end of second vegetation periodc |