Skip to main content

Table 2 Soil microbial characteristics in different freeze–thaw stages

From: Responses of soil microbial communities to freeze–thaw cycles in a Chinese temperate forest

Variables O horizon soil A horizon soil Two-way ANOVA
Stage I Stage II Stage III Stage I Stage II Stage III FTC H FTC × H
MBC (mg·kg−1) 3951.5 ± 407.4ab 4661.8 ± 418.6a 3322.2 ± 395.8b 1925.4 ± 325.7ab 2689.3 ± 463.0a 1493.3 ± 289.6b * *** n.s.
MBN (mg·kg−1) 462.7 ± 60.7 667.4 ± 85.1 467.9 ± 78.0 199.4 ± 38.0b 343.0 ± 57.7a 196.7 ± 42.4b * *** n.s.
MBP (mg·kg−1) 86.6 ± 12.6b 127.5 ± 6.3a 57.2 ± 5.5c 24.8 ± 5.6b 78.8 ± 20.9a 17.6 ± 4.9b *** *** n.s.
MBC/MBN 8.7 ± 0.4a 7.2 ± 0.4b 7.4 ± 0.5b 9.8 ± 0.2a 7.8 ± 0.2b 8.2 ± 0.8b ** * n.s.
MBC/MBP 47.4 ± 4.1ab 36.4 ± 2.3b 58.3 ± 4.8a 81.1 ± 5.9a 39.1 ± 5.0b 93.4 ± 15.9a *** *** n.s.
MBN/MBP 5.4 ± 0.3b 5.2 ± 0.5b 8.1 ± 1.0a 8.3 ± 0.6ab 5.1 ± 0.8b 12.2 ± 2.7a ** * n.s.
BG (nmol h−1 g−1) 42.7 ± 9.9b 63.5 ± 4.6a 30.8 ± 2.2b 12.1 ± 3.4b 29.2 ± 4.7a 14.1 ± 4.0b *** *** n.s.
NAG (nmol h−1 g−1) 12.7 ± 3.1 14.7 ± 1.3 8.9 ± 1.3 4.0 ± 1.0b 10.5 ± 1.8a 5.2 ± 0. 9b ** *** n.s.
ACP (nmol h−1 g−1) 171.9 ± 19.9ab 212.4 ± 17.9a 149.8 ± 11.1b 97.0 ± 17.2 134.1 ± 14.1 98.2 ± 15.1 * *** n.s.
BG/NAG 3.5 ± 0.4 4.4 ± 0.4 3.7 ± 0.5 3.0 ± 0.2 3.0 ± 0.5 2.89 ± 0.6 n.s. * n.s.
BG/ACP 0.24 ± 0.03ab 0.31 ± 0.03a 0.21 ± 0.01b 0.12 ± 0.01b 0.23 ± 0.05a 0.13 ± 0.02ab ** *** n.s.
NAG/ACP 0.07 ± 0.01 0.07 ± 0 .00 0.06 ± 0.01 0.04 ± 0.00b 0.08 ± 0.01a 0.05 ± 0.01ab n.s. n.s. *
Bacteria biomass
(108 copies·g−1)
482.9 ± 60.7b 655.1 ± 24.4a 393.8 ± 58.6b 526.3 ± 25.3ab 612.6 ± 44.0a 430.4 ± 51.0b *** n.s. n.s.
Fungi biomass
(108 copies·g−1)
7.8 ± 1.3 8.4 ± 2.9 8.1 ± 1.6 8.6 ± 1.4 12.5 ± 3.2 10.5 ± 2.3 n.s. n.s. n.s.
F/B ratio 0.016 ± 0.002 0.012 ± 0.004 0.021 ± 0.004 0.017 ± 0.003 0.022 ± 0.007 0.024 ± 0.004 n.s. n.s. n.s.
  1. MBC microbial biomass C, MBN microbial biomass N, MBP microbial biomass P, BG β-glucosidase, NAG β-N-acetyl-glucosaminidase, ACP acid phosphatase; bacteria biomass is represented by the copy number of 16S rRNA; fungi biomass is represented by the copy number of ITS gene; F/B ratio was calculated by the ratio of ITS and 16S rRNA gene copy numbers, the same below. Data are displayed as means ± standard errors (n = 5). The effects of the freeze–thaw cycle (FTC), soil horizon (H), and their interaction (FTC × H) are shown at the right columns. Bold lowercase letters indicate significant differences among different stages within the same horizon (P < 0.05). Two-way ANOVA results *P < 0.05, ** P < 0.01, *** P < 0.001 and n.s. not significant