A spatially distributed, deterministic approach to modeling Typha domingensis (cattail) in an Everglades wetland
© Lagerwall et al.; licensee Springer. 2012
Received: 2 July 2012
Accepted: 7 October 2012
Published: 1 November 2012
The emergent wetland species Typha domingensis (cattail) is a native Florida Everglades monocotyledonous macrophyte. It has become invasive due to anthropogenic disturbances and is out-competing other vegetation in the region, especially in areas historically dominated by Cladium jamaicense (sawgrass). There is a need for a quantitative, deterministic model in order to accurately simulate the regional-scale cattail dynamics in the Everglades.
The Regional Simulation Model (RSM), combined with the Transport and Reaction Simulation Engine (TARSE), was adapted to simulate ecology. This provides a framework for user-defineable equations and relationships and enables multiple theories with different levels of complexity to be tested simultaneously. Five models, or levels, of increasing complexity were used to simulate cattail dynamics across Water Conservation Area 2A (WCA2A), which is located just south of Lake Okeechobee, in Florida, USA. These levels of complexity were formulated to correspond with five hypotheses regarding the growth and spread of cattail. The first level of complexity assumed a logistic growth pattern to test whether cattail growth is density dependent. The second level of complexity built on the first and included a Habitat Suitability Index (HSI) factor influenced by water depth to test whether this might be an important factor for cattail expansion. The third level of complexity built on the second and included an HSI factor influenced by soil phosphorus concentration to test whether this is a contributing factor for cattail expansion. The fourth level of complexity built on the third and included an HSI factor influenced by (a level 1–simulated) sawgrass density to determine whether sawgrass density impacted the rate of cattail expansion. The fifth level of complexity built on the fourth and included a feedback mechanism whereby the cattail densities influenced the sawgrass densities to determine the impact of inter-species interactions on the cattail dynamics.
All the simulation results from the different levels of complexity were compared to observed data for the years 1995 and 2003. Their performance was analyzed using a number of different statistics that each represent a different perspective on the ecological dynamics of the system. These statistics include box-plots, abundance-area curves, Moran’s I, and classified difference. The statistics were summarized using the Nash-Sutcliffe coefficient. The results from all of these comparisons indicate that the more complex level 4 and level 5 models were able to simulate the observed data with a reasonable degree of accuracy.
A user-defineable, quantitative, deterministic modeling framework was introduced and tested against various hypotheses. It was determined that the more complex models (levels 4 and 5) were able to adequately simulate the observed patterns of cattail densities within the WCA2A region. These models require testing for uncertainty and sensitivity of their various parameters in order to better understand them but could eventually be used to provide insight for management decisions concerning the WCA2A region and the Everglades in general.
The Everglades, commonly known as the “River Of Grass” Douglas (1947), in southern Florida, USA, once covered some 28,500 km2. This wetland ecosystem was sustained by the Kissimmee River, flowing through Lake Okeechobee and southwards as a shallow, slow-moving sheet of water flowing freely to the estuaries of Biscayne Bay, Ten Thousand Islands, and Florida Bay. The channelization of the Everglades around 1948 caused the reduction of the original wetland areas by up to 50%, with related declines in dependent wildlife. In addition to the changes in hydrology, continuous mining, agriculture, and urbanization activities have resulted in invasive and exotic plants becoming established in place of the original vegetation, altering habitats and often forming mono-crop stands (single species environments) (Odum et al. 2000).
The Comprehensive Everglades Restoration Plan (CERP) was implemented in 2000 (USACE, S.F.R.O 2010a) with the explicit goal of restoring some of the Everglades’ former extent and ecosystem functioning. The main focus of CERP has been on improved management of water quantity and water quality with the assumption that if the water quantity and quality are adequate, the ecology will follow suit. There is, however, an increasing focus on the ecological impacts of various management decisions, and these efforts center on improving species diversity and protecting existing habitats (USACE, S.F.R.O 2010b). In an effort to achieve these goals, stormwater treatment areas (STA) were constructed just south of the Everglades agricultural area (EAA) to filter out phosphorus from the water before releasing it into the water conservation areas (WCA). The WCAs act as impoundments for water storage and flood control as well as serving as wildlife habitat. Water flows from these WCAs into the Everglades National Park (Guardo et al. 1995).
Typha domingensis as an invasive species
The emergent wetland species Typha domingensis (cattail) is a native Everglades monocotyledonous macrophyte, typically occurring as a sparse complement alongside Cladium jamaicense (sawgrass) stands. These two species have significantly different morphology, growth, and life history characteristics (Miao and Sklar 1998), and this has enabled the cattail to expand prolifically under the altered conditions in the Everglades. In the 1980s, the area covered by cattail stands in WCA2A doubled, expanding southward into the sawgrass marshes (Willard 2010). Cattail has hence been labeled as an indicator species, or species of concern, and its distribution is used to determine the effectiveness of various water management decisions. Cattail expansion has been studied extensively (Miao 2004; Wu et al. 1997; Newman et al. 1998), and it has been determined that there are four main external factors that affect its growth and aid in cattail’s dominance over sawgrass. These factors include water depth, hydroperiod, soil phosphorus concentration, and disturbance (Newman et al. 1998). It was determined that the optimum water depth at which cattail grows is between 24 and 95 cm (Grace 1989), with a hydroperiod of 180–280 days (Wetzel 2001). In terms of soil phosphorus concentration, cattail has been found to be invading the natural sawgrass habitats of WCA2A along a soil phosphorus gradient running from the northwest (high concentrations) to the southeast (low concentrations). Urban et al. (1993) mention that, given an adequate water depth, soil phosphorus concentration is the next most important factor in determining cattail expansion/invasion. In creating their water quality model for simulating soil phosphorus concentrations downstream of the Everglades STAs, Walker and Kadlec (1996) determined that the lower bound soil phosphorus concentration for the optimum growth of cattail was 540 mg/kg. Fires and other disturbances such as hurricanes were also found to affect the colonization of areas by cattail by altering local topography and nutrient concentrations (Newman et al. 1998).
Ecological model designs to address everglades systems
In order to assess these various influences on cattail and other ecological components, a variety of computation models were designed and implemented. These models aid our understanding of complex systems and allow scientists and managers to evaluate different ecological outcomes of decisions before the more costly task of their implementation (Fitz et al. 2011). To ensure numerical efficiency, most spatially distributed models have their equations, laws, and assumptions “hard-coded” into their programming code. This creates a “fixed-form” model, with changes in the functioning coming through extensive code re-writes and careful redesign around logical structures. Dynamic “free-form” simulation models, such as STELLA (Costanza and Voinov 2001), QnD (Kiker and Linkov 2006; Kiker et al. 2006), and the Kepler system (Ludascher et al. 2006) are generally written using an object-oriented programming (OOP) language such as C++ (Stroustrup 2000) or Java (Arnold and Gosling 1998), as opposed to a linear language such as FORTRAN (Cary et al. 1998). When interacting with free-form models and their algorithms, designers do not interact directly with the program code. Rather, they influence objects through placing data, storage, and logical structures into either a graphical user interface (STELLA, Kepler) or within a meta-code structure such as the eXtensible Markup Language (XML) (Harold 1998).
There are a number of fixed-form ecological models currently in use across the Everglades region. Of these, the Across Trophic Level System Simulation (ATLSS) (Gross 1996) and the Everglades Landscape Model (ELM) (Fitz and Trimble 2006b) are probably the most well-known. These and most other models available for modeling cattail in the Everglades are entirely qualitative, that is, they involve switching between one species and another. The majority of these current ecological models are also stochastic, that is, based on probabilities and a degree of randomness and uncertainty. They generally run as post-process models, using hydrological data output by other models such as the South Florida Water Management Model (SFWMM) (Fitz et al. 2011).
The ATLSS vegetation succession model is used to determine the succession of one habitat type to another (e.g., sawgrass to cattail). The ATLSS model simulates with an annual time step on square 500 m cells and uses a stochastic cellular automata model to switch between vegetation types. Currently there is no way to determine vegetation densities within vegetation types (Duke-Sylvester 2005).
The ELM model uses a counter to switch between species by accumulating days of water level and soil phosphorus concentration above certain limits. The model then switches between species based on their preferred hydroperiod and historical soil phosphorus concentrations (Fitz and Trimble 2006a). The ELM model is the only currently available simulation tool for evaluating water quality across the Everglades landscape and does not simulate detailed ecological features (Fitz et al. 2011).
Another modeling effort by Wu et al. (1997) used Markov chain probabilities to switch between Cladium and Typha species. This model was in fact used to inform the ATLSS nutrient and fire disturbance model (Wetzel 2003). Again, this is a stochastic, species-specific, presence/absence-type model.
A modeling effort by Tarboton et al. (2004) developed a set of habitat suitability indices (HSI) for evaluating water management alternatives. These HSIs provided a range of probabilities for a particular species occurring across the landscape and were based predominantly on local hydrological conditions such as depth (maximum, minimum, and mean), hydroperiod, velocity, and flow direction.
Given that water quantity (depth) and quality (soil phosphorus concentration) affect cattail (and other plants) growth and distribution, there is a need to integrate these components to determine the more detailed biological outcomes of an Everglades ecological model. There is also a need for a quantitative model to provide continuous density values for specific vegetation rather than simply presence/absence information. Given that the Everglades restoration includes a large and ongoing research effort, there is a need to efficiently test and explore potentially useful algorithms in an adaptable, ecological modeling engine.
The RSM/TARSE ecological model
A combined effort of the University of Florida, the South Florida Water Management District (SFWMD), and the US Geological Survey created the Transport and Reaction Simulation Engine (TARSE) (Jawitz et al. 2008), which was originally designed to run in line with the SFWMD-developed Regional Simulation Model (RSM) (SFWMD 2005c) to simulate soil phosphorus dynamics in the Everglades system. The OOP structure of this coupled hydrologic/water quality model, along with the user-definable inputs and interactions, allowed for the extension of this model beyond its original purpose into ecological processes and features. The coupled RSM/TARSE (henceforth referred to as RTE) model, implemented with the goal of modeling ecological features within the southern Florida landscape and presented in this paper, is a spatially distributed, free-form model simulating cattail biomass distribution and dynamics across WCA2A. Using the RTE model to couple vegetation dynamics with phosphorus dynamics has been alluded to by Jawitz et al. (2008), Muller (2010), and Perez-Ovilla (2010) during their respective TARSE-influenced, WQ simulations. Zajac (2010) used vegetation types to influence Manning’s n and evapotranspiration coefficients. These parameters were informed by initial vegetation types and not by changing vegetation distribution and density over time.
There is therefore a definite need for the RTE model, which allows one to model a vegetation species quantitatively and ultimately determine the ecological impact of various management scenarios falling under the CERP initiative. This new engine would accommodate different algorithms or new species as available data or new knowledge becomes available. It would allow for interactions and feedback effects within species as well as among different species and with other environmental factors.
Objectives and hypotheses
Whether cattail growth is density dependent.
Whether water depth is an important factor for cattail expansion.
Whether soil phosphorous is a contributing factor for cattail expansion.
Whether sawgrass density impacts the rate of cattail expansion.
Whether inter-species interactions between cattail and sawgrass contribute to the observed cattail dynamics.
Following the methodology used by Jawitz et al. (2008), a simple logistic function (Keen and Spain 1992) formed the base of the complexities with water depth and soil phosphorus concentration [the two most important factors influencing cattail growth according to Newman et al. (1998)] and sawgrass interaction influencing the higher levels of complexity. A second step in our objective was to use an existing ecosystem and its monitoring data to analyze performance of our five candidate models. The entire WCA2A vegetation dataset (1991, 1995, and 2003), obtained from Rutchey et al. (2008), was chronologically divided into model training and testing sections. Training of the model was conducted for the years 1991–1995, where the growth factor (found in Equation 3) was fitted to the level 1 complexity. As a third step in our objective, model testing was conducted on the two time periods of 1991–2003 (testing 1) and 1995–2003 (testing 2), respectively, with the testing 2 time period being equivalent to a blind test (due to different initial conditions). The 1991 and 1995 vegetation maps were used to initialize the training, testing 1, and testing 2 simulations, respectively. Model output from the training, testing 1, and testing 2 simulations was compared with the 1995 and 2003 vegetation maps. Model output was compared to observed patterns, and the most accurate level of complexity thus determined.
In order to reproduce the observed cattail patterns, both hydrological and water quality data were used as inputs for the ecological model. To this end, it was decided to use the Regional Simulation Model (RSM), which was developed by the South Florida Water Management District (SFWMD) to replace the popular SFWMM, coupled with the Transport and Reaction Simulation Engine (TARSE) to provide the base structure for modeling cattail dynamics across the test site.
The Regional Simulation Model (RSM)
Developed by SFWMD, the RSM simulates hydrology over the South Florida region. It is often thought of as the successor to the successful SFWMM, referred to as the “2-by-2” model for its 2 mile resolution (SFWMD 2005a). The RSM operates over a variable triangular mesh grid, in contrast to the 3.22 km (2 mile) square grid of the SFWMM; this enables higher resolution in areas of concern as well as the ability to delineate canals (SFWMD 2005c). The RSM uses a weighted, implicit, finite volume method to simulate two-dimensional diffusional flow and hence implicitly simulates groundwater flow and surface water flow (SFWMD 2005c). The OOP design structure of RSM allows for the abstraction and modularity of various components (SFWMD 2005b). A result of this is that there are two engines that comprise the RSM, namely the Hydrologic Simulation Engine (HSE) and the Management Simulation Engine (MSE). The HSE simulates all the hydrological processes, while the MSE simulates various management or control regimes. These two engines interact at runtime to provide an accurate representation of the hydrodynamics of the region (SFWMD 2005c).
Simulating transport and reactions using TARSE
Where t is time [T], c(x,t) is the concentration [M/L3], and Φ(x,t) is the porosity of the medium (which may be 1 for surface water) [L3/L3]. h(x,t) is the water depth [L] or thickness of the saturated zone in groundwater flow, u(x,t) is the specific discharge [L/T] of water (either surface or groundwater), and D * =D * (u(x,t)) is the dispersion tensor (a function of u). f 1 (x,t) is a source rate [M/L3.T] with associated concentration c 1 , and f 2 (x,t) is a first-order decay rate [M/L3.T]. The density [M/L3] of the water is assumed to be constant.
The main equations are structured as ordinary differential equations (ODE) (SFWMD 2008a).
The RSM/TARSE coupling represents possibly the first time that a free-form dynamic system model has been integrated with a fixed-form, spatially distributed, hydrologic model (Muller 2010). This unique coupling, with user-defined interactions operating across a spatially distributed domain, lends itself to simulating ecological behaviors (growth, death, movement, and feeding) as well as the original WQ interactions. The model can currently only solve ADRE movement and as such is insufficient for ecological/animal movement. Attempts to include some form of Lagrangian-type movement in this model are discussed by Lagerwall (2011).
Where P is the population density [M/L2], t is time [T], GF is the constant growth rate [T-1], and K is the carrying capacity or maximum population density [M/L2].
Where P is the population density [M/L2], t is time [T], GF is a constant growth rate [T-1], DepthF is the water depth factor [L/L], K is the carrying capacity or maximum population density [M/L2].
Where phosphorusF is the soil phosphorus HSI, ranging from 0 to 1, and phosphorus is the current soil phosphorus concentration (mg/kg).
Where sawgrassF is the sawgrass HSI ranging from 0 to 1, sawgrass is the current sawgrass density, and K SAW is the sawgrass carrying capacity.
Where cattailF is the cattail HSI ranging from 0 to 1, cattail is the current cattail density, and K CAT is the cattail carrying capacity.
The depth, soil phosphorus, and sawgrass interaction factors are all calculated using the pre-equations, similar to that presented in Equation 2. These factors are then incorporated into the main growth equations, presented in Equations 4, 5, 7 and 9 representing levels of complexity 2 through 5, respectively.
In TARSE, components are listed as either mobile or stabile. Mobile components are moved in the water using the ADRE equations, while the stabile components do not move and only undergo the reaction part of the ADRE. Given the complexities associated with simulating wind-borne or water-borne transportation of seeds and rhizome expansion—which is another mode of expansion noted by Miao (2004)—all mesh elements were initialized (seeded) with cattail, with areas originally not containing cattail being seeded with the minimum value of 10 g(dry weight)/m2. This assumption represents the presence of a seed bank, providing cattail the opportunity to colonize an area as soon as conditions become favorable. Vegetation then is modeled as a stabile component, with no means for dispersal, or in another way we assume “infinite dispersal.” The latter assumption is supported by very high values of dispersal for seeds in the Everglades, enhanced by the diffused presence of biotic (animals) and abiotic (water, wind) dispersal vectors (Miao and Sklar 1998). Also, as a result of this current inability for modeled dispersal, the maximum influence that the aforementioned factors such as phosphorusF, sawgrassF, and cattailF can have has been limited so that they reduce the cattail population to 1% of its maximum density.
Initial conditions, boundary conditions, and time series data
Hydrological time series
The hydrology of WCA2A is controlled primarily by the operation of control points along the S10 and L35-B canals. The hydrology data were obtained from the SFWMD, which uses the WCA2A site as a test site for the RSM. The average depth for the region ranges from 60 to 90 cm (SFWMD, 2008c). The input dataset consisted of a daily time series of hydraulic head values (m) at the inlet and outlet control structures of WCA2A (represented by the green squares in Figure 1) for the years 1979–2000 (Wang 2009). The time series have since been updated to 2008 for all control structures using data collected from the DBHYDRO website (SFWMD 2009).
A gradient of soil phosphorus exists along WCA2A, with a high concentration near the inlets at the north, and a low concentration at the outlets in the south. This soil phosphorus gradient has been widely documented and studied (DeBusk et al. 1994; Grunwald et al. 20042008; Rivero et al. 2007a,b; Grunwald 2010). Given the unavailability of spatial soil phosphorus data beyond map classifications (Grunwald 2010), soil phosphorus input maps were created by overlaying the WCA2A mesh on the existing maps obtained from Grunwald et al. (20042008). The soil phosphorus map of 1990 was used for the model training period of 1991–1995, while the soil phosphorus map of 2003 was used for both the testing 1 (1991–2003) and testing 2 (1995–2003) simulation periods. Due to the poor quality of these soil phosphorus input maps and the inability of TARSE to adequately simulate phosphorus dynamics in the WCA2A region (as it is still in development), the soil phosphorus concentration itself was not simulated, i.e., the static soil phosphorus concentration provided by the input maps was used to inform the model throughout the simulation period.
Cattail and sawgrass
Vegetation maps for WCA2A were obtained for the years 1991, 1995 (Rutchey 2011), and 2003 (Wang 2009), which were all used in Rutchey et al. (2008). These maps provided density (g/m2) distributions across the test site for cattail. The negative correlation between sawgrass and cattail has been reported by Doren et al. (1999) and Richardson et al. (2008), and various other vegetation maps of the area, namely 1991 (Jensen et al. 1995), 1995 (SFWMD 1995), 1999 (SFWMD 1999), and 2003 (Wang 2009), confirm this negative correlation. Although sawgrass density is related to more environmental factors than only cattail density (Miao and Sklar 1998), a simple negative correlation with the cattail maps was used in order to assign densities to the sawgrass maps. For example, high sawgrass density values (1,600 g/m2) were assigned to regions with typically low cattail density values, and low sawgrass density values (600 g/m2) were assigned to regions with high cattail density values.
Cattail class and density values for formatting data maps
Cattail density value (g/m2)
Sawgrass density value (g/m2)
1 - High density cattail
2 - Medium density cattail
3 - Low density cattail
4 - Other
Parameter description for the increasing levels of complexity studied
Cattail growth rate
Rate of increase of population
Water depth influence
Cattail carrying capacity, Cattail
Soil phosphorus concentration influence
Cattail carrying capacity, cattail
Sawgrass, cattail carrying capacity, cattail
Sawgrass growth rate
Rate of increase of population
Statistical analysis of simulated and monitored biomass
Where E f is the Nash-Sutcliffe coefficient, is the predicted variable, y i is the observed variable, is the mean of the observed variable, and n is the sample size. A Nash-Sutcliffe value of 1 means that the model completely matches the data, while a value of 0 means that the model performs no better than the mean of the data. Any value less than 0 is interpreted as a poor representation of the data.
A direct comparison between model output and the data was performed with the use of a classified difference technique (Kiker 1998). Since the data maps were initialized with a minimum density of 10 g/m2 to account for movement between triangular elements that is not simulated in this model application, a difference between model output and the data value falling within 20 g/m2 was considered a “perfect” match. This is loosely based on the fact that Miao and Sklar (1998) reported a roughly 10% error in measurement of the maximum density of 1,240 g/m2. So, for example, if the data value was 10 g/m2 (representing a typical non-cattail region), and the model output was 12 g/m2, with a difference of 2 g/m2 (falling within the 20 g/m2 range), then this would be considered a “perfect” match. The next class of differences lies within the 200 g/m2 range, which is the value assigned to the low cattail density class during the formatting and creation of the input data maps. This 200 g/m2 range is also half the range between the successively higher cattail density classes. The third class of differences lies within 400 g/m2, which can be thought of as a data class difference (e.g., between low and medium densities) or also as being within 40% of the maximum possible difference (the maximum data density is set as 1,000 g/m2). Finally, any difference above the 400 g/m2 threshold is placed in the fourth class of differences and represents a significant misrepresentation of the data by the model.
A box and whiskers plot (Ott and Longnecker 2004) was created with all model element values compared with their corresponding data element values. The desired figure is a plot with the means and ranges corresponding to the associated data ranges. The box and whiskers plots cover the entire range of possible values from 0 to 1,240 g/m2.
Where x i is the current cell value, x j is the value of the cell separated by a given distance, x(bar) is the mean, and W is the number of cells surrounding the current one and found within the given distance. These values are plotted against an increasing cell-pairwise distance, as in Marani et al. (2006), to determine the trend in spatial autocorrelation across the entire region.
A landscape-scale abundance-area plot (Martin 1980; Michalski and Peres 2007) was used to measure the average change in density across the test site. One hundred randomly distributed cells are used as base cells. From these, the densities of all cells falling within a given radius are summed. This total is then divided by the number of base cells and plotted against the area of circles with an increasing radius as in Martin (1980).
A trend in the regional mean density was plotted with a daily timestep for a visual comparison of the trends between the different levels of complexity. This was repeated for the individual levels of complexity and selected zones (elements) within the region, for a more detailed view of the effect of external parameters on different areas of the region. Elements 209, 244, and 380, located in the northeast, central, and southwest, were selected as representative elements for typically high, medium, and low cattail densities, respectively. These elements are marked by red squares in Figure 1 and are useful for evaluating local vegetation indicators.
Model training and testing
There were three time periods over which the model was simulated using the available data maps of 1991, 1995, and 2003. Training was performed for the time period 1991–1995 using the level 1 complexity to establish the growth rate (6.7 × 10-9 g/g.s), and results from the other levels will be due solely to the effect of their included external parameters. It is therefore expected that the results of the other levels of complexity will not be as accurate as the level 1 complexity for this time period. Testing of the model was performed for the time period 1991–2003. This provides an extended forecast based on the original calibration time period and initial data. Finally the 1995–2003 time period was used as a blind test of the model, using different initial conditions and determining its ability to accurately predict the density distribution of the 2003 cattail map.
Results and discussion
From the cattail maps of Figure 2 and those in Rutchey et al. (2008), a trend in cattail distribution over the years is observable. It appears that cattail density and distribution increased from 1991 to 1995. From 1995 to 2003 the general distribution continued to increase but with more dispersed patches of high-density cattail. This may be related to a reduction in the overall dispersal or to an increased local speciation. Through the use of best management practices, the total phosphorus load entering WCA2A for the period 1995–2004 was reduced by roughly 36% (Richardson et al. 2008), which may have also had a role in the dispersal noted above.
When considering the first hypothesis, or level of complexity, that cattail growth is density dependent, we note the following points. For the training (1991–1995) time period, the level 1 complexity’s spatial density distribution (Figures 4 and 9) is the most similar to the observed 1995 data. The density trend (Figure 5) is smooth and slowly increasing for all observed points (red dots). The regional trend ends directly on the data density. The southwest (element 380) and central (element 244) trends over-predict the data points. The abundance-area statistic (Figure 6b) follows the data trend (black line) the closest. The mean and distribution of densities (Figure 6c) are relatively close to the data. The Moran’s I statistic follows the data (black line) trend relatively closely (Figure 6d). All of these results from the training period are expected because this level of complexity was used for calibration over this time period. For the two testing simulation periods, the level 1 complexity clearly overestimates the historical data (Figures 4 and 9). The density trend (Figure 5b,c) remains smooth but overestimates the observed data, except for element 380 in Figure 5c which remains low, possibly due to the low initial starting density and relatively short time period. The abundance-area statistic (Figures 7b and 8b) shows significant over-prediction of the data trend (black line). The mean density is still low, but the distribution is significantly skewed toward the higher densities (Figures 7c and 8c). This is evidence that a spatial distribution of densities is more informative than simply using the mean for the area or a presence/absence type model. Moran’s I statistic follows the data (black line) trend relatively closely (Figures 7d and 8d). The results of these analyses confirm that although cattail may indeed have a density-dependent/logistic growth pattern as we are able to simulate observed data during the training period, our inability to simulate observed data for the two training periods indicates that there are certainly other parameters affecting the growth and distribution of this species.
When considering the second hypothesis, or level of complexity, that cattail growth/expansion is dependent on water depth, we note the following points. For all time periods (training, testing 1, and testing 2), the level 2 complexity’s spatial density distribution (Figures 4 and 9) is consistently lower than the observed values. This is confirmed in the trend analysis (Figure 5a,b,c), where all the observed elements (209, 244, and 380) and the regional trend are consistently below the observed values. The only exception is element 380 in Figure 5a, where there is hardly any change in the element’s density, and this is possibly due to the low initial density value of that element. The abundance-area statistic for all time periods (Figures 6b, 7b, 8b) is significantly lower than the observed trend. Similarly, the distribution of densities for all time periods (Figures 6c, 7c, 8c) is much reduced. For the Moran’s I statistic, the model is relatively close to the data trend but consistently has a longer (the longest) tail. This implies that cells further away have an observable impact on the density of any other cell. This would be due to the fact that the water depth in every cell has an effect/influence on every other cell in the region. We know that water depth is an influential factor in cattail growth (Newman et al. 1998; Miao and Sklar 1998), however the results of these analyses indicate that the current model (level 2 complexity) is overly influenced by this parameter. It is expected that the influence of this parameter will be reduced as it is “diluted” with other parameters in the higher complexity models.
When considering the third hypothesis, or level of complexity, that cattail growth/expansion is dependent on soil phosphorus concentration, we note the following points. The spatial density distribution (Figures 4 and 9) for level 3 lies somewhat in-between that for level 1 and level 2. Except for the training period, which slightly under-predicts the observed values, the two testing periods appear to more accurately predict the observed density distribution. This is confirmed with the trend analysis (Figure 5a,b,c), where at least the regional trend is at or relatively close to the observed values. As with the level 2 complexity, element 380 tends to under-predict the observed value. However, element 209 tends to predict the observed value better than either of the previous two levels of complexity. The abundance-area statistic (Figures 6b, 7b, 8b) shows consistent under-prediction of the observed trend, but also shows consistently higher values than the level 2 trend and is closer to the data than the level 1 trend. The distribution of densities for all time periods (Figures 6c, 7c, 8c), although greater than the level 2 complexity, is still significantly lower than the observed distribution. The Moran’s I trend is followed closely for all time periods (Figures 6d, 7d, 8d). The results of these analyses confirm that soil phosphorus is a significant influencing factor in the distribution of cattail, although the water depth parameter remains highly influential. The level 3 complexity is better able to predict cattail in areas of typically high phosphorus or of high cattail density than the previous two levels of complexity.
When considering the fourth hypothesis, or level of complexity, that sawgrass density may impact the rate of cattail expansion, we note the following points. The spatial density distribution (Figures 4 and 9) is closer to the observed values than the previous levels of complexity. This is confirmed in the trend analysis (Figure 5a,b,c), where most notably all of the elements tend to better predict the observed values, except for element 244 in Figure 5c, which over-predicts the observed density and in turn raises the regional trend above the observed value as well. The abundance-area statistic only slightly under-predicts the observed trend during the training time period (Figure 6b). During the two testing time periods, the statistic indicates a slight over-prediction of the observed trend, but results show better predictions than any of the previous levels of complexity. The density distribution (Figures 6c, 7c, 8c) is significantly higher than the level 2 and level 3 complexities, and equal to (Figure 6c; training) or less than (Figures 7c, 8c; testing) the level 1 complexity. This means that the level 4 complexity consistently approximates the observed densities for the region better than the other levels of complexity for all time periods, albeit with slightly elevated minimum densities. The Moran’s I statistic (Figures 6d, 7d, 8d) follows the observed trend relatively well for all time periods. Although the level 4 complexity tends to have slightly elevated minimum densities, like the level 1 complexity, the general result from these analyses is that the level 4 complexity is able to simulate the cattail densities through the region consistently better than any of the previous levels of complexity. We can thus conclude that including a simulated sawgrass density does indeed impact the rate of cattail expansion and improve simulation results.
When considering the fifth hypothesis, or level of complexity, that inter-species interactions between cattail and sawgrass contribute to the observed cattail dynamics, we find the following: The spatial density distribution (Figures 4 and 9) does not predict the observed values significantly better than the level 4 complexity. The trend analysis (Figure 5a,b,c) is almost identical to that of the level 4 complexity in every respect. All of the statistical analyses and distributions for all time periods (Figures 6b,c,d; 7b,c,d; 8b,c,d) are almost identical to those of the level 4 complexity. The result of these analyses is that the level 5 complexity does not predict the observed values with greater success than the level 4 complexity. While inter-species interactions might well have an effect with a different model structure, the current modeling arrangement has shown the beginning of diminishing returns with respect to model complexity and predictive capability.
With regard to the Moran’s I statistic, all the complexity levels followed the same basic trend as the data (represented by the black line) and were all 0 by around the 18,240 m mark. This distance corresponds approximately to the width of the region, while the total distance of 36,480 m in the plot corresponds to the longest north–south distance of the region. It is believed that the statistic drops to 0 by the 18,240 m mark due to overlapping and boundary effects and that this elevates the Nash-Sutcliffe coefficient for all levels of complexity in this statistic.
Summary of Nash-Sutcliffe values comparing model and observed data for box plot, Moran’s I , and abundance-area statistics (represented by Figures 6 , 7 , and 8 , respectively) for level 1, level 2, level 3, level 4, level 5, training (199–1995), testing 1 (1991–2003), and testing 2 (1995–2003) simulations
1-to-1 Box plot
The methods of modeling cattail for ecological models currently in use were compared, their similarities and differences were noted, and a knowledge gap identified: there doesn’t yet exist a method of quantitatively and deterministically determining the spatial distribution of cattail in the Everglades. A coupled free-form/fixed-form model was introduced to solve this problem. An added benefit of the free-form nature of the RSM/TARSE coupled model is the user-definable equations of interaction, which can be modified as data and/or new theories become available. This new ecological implementation of the model (RTE) was successfully applied towards modeling cattail dynamics across the WCA2A test site for training (1991–1995), testing (1991–2003), and blind test (1995–2003) simulation periods. Five algorithms, with increasing complexity, were used to match the historical data. Upon analysis of the performance of these different levels, it can be concluded that the level 4 and 5 complexities, which include depth, soil phosphorus, and sawgrass interaction parameters, are the most suitable models for matching the historical data. The Nash-Sutcliffe coefficient was used to distinguish the success of different models.
Both local and landscape-scale indicators were used to perform the comparison between historical and modeled cattail patterns. The average local cattail density was estimated with a box-plot analysis; the pairwise-cell comparison of local cattail densities was analyzed with Moran’s I; and, the regional increase with area of the local cattail density was estimated through the abundance-area relationship. The box-plot and the abundance-area were the most meaningful patterns to discriminate models in terms of their ability to represent the observed patterns.
The autocorrelation structure of the cattail patterns were well represented by all the models at each complexity level. This is possibly due to the fact that through overlapping and boundary effects, cattail densities leveled off after roughly half the distance (top to bottom) that was used to calculate the statistic. It may be more representative if future calculations considered only half this maximum distance, where the variations would carry a greater weighting.
Our simulation results would be in agreement with the studies of Newman et al. (1998) and Miao and Sklar (1998), in which water depth and soil phosphorus concentration were the most important factors aiding in cattail expansion. Our results also include an interaction parameter with sawgrass, which is of interest in the region. Thus, we confirm the importance of considering species dependencies or interactions in reproducing the cattail patterns even in water-controlled areas in which the anthropic-driven variables would be expected to dominate the species processes and the resulting patterns.
Limitations of our current modeling approach may include the element/triangle size, with a range of 0.5–1.7 km2 (Wang 2009). This constraint was dictated by the choice of the RSM that simulates hydrological processes. Although the imposed grid-unit has a relatively coarse size in which there is still considerable heterogeneity of the environmental features (Zajac 2010), RTE has proven to be capable of reproducing the dynamics of cattail and sawgrass at the landscape scale using the level 4 and level 5 complexities. This makes it a valuable tool for exploring potential management scenarios in water conservation areas in the Everglades and possibly in other water-controlled wetlands.
Further investigations would consider the quantification of the importance of water-controlled drivers and species traits (dispersal) for vegetation patterns, the stability/instability states of species under varying stressors, the prediction of future management scenarios, and the comparison with neutral-based models.
In terms of further model development and added complexity, efforts have been made towards more accurate representation of fauna movement through the use of Eulerian–Lagrangian (grid-independent) particle movement (Lagerwall 2011), as well as using vegetation types/densities to influence the hydrology with a dynamically linked Manning’s n parameter (Zajac 2010). While creating more dynamically linked parameters is an ongoing task, these linkages remain a challenge to implement due to the difficulties associated with parameterizing (training) a model with feedback effects. This feedback relationship between ecological and hydrological model components may be quite important to the function and resilience of these ecosystems and is certainly a subject of further research.
Financial support for this research was provided by the South Florida Water Management District and the U.S. Geological Survey - Water Resources Research Center at the University of Florida.
- Arnold K, Gosling J: The Java programming language. 2nd edition. Prentice Hall, Upper Saddle River, NJ; 1998.Google Scholar
- Cary JR, Shasharina SG, Cummings JC, Reynders JVW, Hinker PJ: Comparison of C++ and Fortran 90 for object-oriented scientific programming. Comp Phys Comm 1998, 105: 20–36.View ArticleGoogle Scholar
- Cliff AD, Ord K: Spatial autocorrelation: a review of existing and new measures with applications. Econ Geography 1970, 46: 269–292.View ArticleGoogle Scholar
- Convertino M, Muneepeerakul R, Azaele S, Bertuzzo E, Rinaldo A, Rodriguez-Iturbe I: On neutral metacommunity patterns of river basins at different scales of aggregation. Water Resour Res 2009, 45: W08424.View ArticleGoogle Scholar
- Costanza R, Voinov A: Modeling ecological and economic systems with STELLA: part III. Ecol Model 2001, 143: 1–7. 10.1016/S0304-3800(01)00358-1View ArticleGoogle Scholar
- DeBusk WF, Reddy KR, Koch MS, Wang Y: Spatial distribution of soil nutrients in a northern-Everglades marsh: Water Conservation Area 2A. Soil Soc Am 1994, 58: 543–552. 10.2136/sssaj1994.03615995005800020042xView ArticleGoogle Scholar
- Doren RF, Armentano Thomas V, Whiteaker Louis D, Jones Ronald D: Marsh vegetation patterns and soil phosphorus gradients in the Everglades ecosystem. Aqua Bot 1999, 56: 145–163.View ArticleGoogle Scholar
- Douglas MS: The Everglades: river of grass. Rinehart, New York; 1947.Google Scholar
- Duke-Sylvester S: Initial performance measures and information related to the ATLSS vegetation succession model. 2005. . Accessed 31 July 2010 http://atlss.org/VSMod . Accessed 31 July 2010Google Scholar
- ESRI (Environmental Systems Resource Institute): ArcMap 10.0. ESRI, Redlands, CA; 2010.Google Scholar
- Fitz CH, Trimble B: Documentation of the Everglades Landscape Model: ELM v2.5. South Florida Water Management District, West Palm Beach, FL; 2006a.Google Scholar
- Fitz CH, Trimble B: Everglades Landscape Model (ELM). 2006b. . Accessed 31 July 2010 http://my.sfwmd.gov/portal/page/portal/xweb%20-%20release%202/elm . Accessed 31 July 2010Google Scholar
- Fitz HC, Kiker GA, Kim JB: Integrated ecological modeling and decision analysis within the Everglades landscape. Crit Rev Environ Sci Technol 2011,41(S1):517–547.View ArticleGoogle Scholar
- Fortin MJ, Dale MRT: Spatial analysis, a guide for ecologists. Cambridge University Press, Cambridge; 2005.Google Scholar
- Grace JBL: Effects of water depth on Typha latifolia and Typha domingensis. Am J Bot 1989, 76: 762–768. 10.2307/2444423View ArticleGoogle Scholar
- Gross LJ: ATLSS home page. 1996. . Accessed 31 July 2010 http://atlss.org/ . Accessed 31 July 2010Google Scholar
- Grunwald S: Phosphorus data for WCA2A. Personal Communication. University of Florida, Gainesville; 2010.Google Scholar
- Grunwald S, Reddy KR, Newman S, DeBusk WF: Spatial variability, distribution and uncertainty assessment of soil phosphorus in a South Florida wetland. Environmetrics 2004, 15: 811–825. 10.1002/env.668View ArticleGoogle Scholar
- Grunwald S, Ozborne TZ, Reddy KR: Temporal trajectories of phosphorus and pedo-patterns mapped in Water Conservation Area 2, Everglades, Florida, USA. Geoderma 2008, 146: 1–13. 10.1016/j.geoderma.2008.03.023View ArticleGoogle Scholar
- Guardo M, Fink L, Fontaine Thomas D, Newman S, Chimney M, Bearzotti R, Goforth G: Large-scale constructed wetlands for nutrient removal from stormwater runoff: an Everglades restoration project. Environ Manage 1995,19(6):879–889. 10.1007/BF02471939View ArticleGoogle Scholar
- Harold ER: XML: Extensible Markup Language. 1st edition. IDG, Foster City; 1998.Google Scholar
- James AI, Jawitz JW: Modeling two-dimensional reactive transport using a Godunov-mixed finite element method. J Hydrol 2007, 338: 28–41. 10.1016/j.jhydrol.2007.02.007View ArticleGoogle Scholar
- Jawitz JW, Muñoz-Carpena R, Muller S, Grace KA, James AI: Development, testing, and sensitivity and uncertainty analyses of a Transport and Reaction Simulation Engine (TaRSE) for spatially distributed modeling of phosphorus in South Florida peat marsh wetlands. Scientific Investigations Report 2008–5029. United States Geological Survey, Reston, VA; 2008.Google Scholar
- Jensen JR, Rutchey K, Koch MS, Narumalani S: Inland wetland change detection in the Everglades Water Conservation Area 2A using a time series of remotely sensed data. Photogramm Eng Rem Sens 1995,61(2):199–209.Google Scholar
- Keen RE, Spain JD: Computer simulation in biology. Wiley-Liss, New York; 1992.Google Scholar
- Kiker GA: Development and comparison of savanna ecosystem models to explore the concept of carrying capacity. PhD Dissertation. Cornell University, Ithaca; 1998.Google Scholar
- Kiker GA, Linkov I: The QnD Model/Game System: Integrating Questions and Decisions for Multiple Stressors. Springer, Netherlands; 2006.Google Scholar
- Kiker GA, Rivers-Moore N A, Kiker M K, Linkov I: QnD: A modeling game system for integrating environmental processes and practical management decisions. Environmental Security and Environmental Management: The Role of Risk Assessment. Netherlands 2006.Google Scholar
- Lagerwall GL: Modeling Typha domingensis in an Everglades wetland. Dissertation. University of Florida, Gainesville; 2011.Google Scholar
- Lindenschmidt KE: The effect of complexity on parameter sensitivity and model uncertainty in river water quality modeling. Ecol Model 2006, 190: 72–86. 10.1016/j.ecolmodel.2005.04.016View ArticleGoogle Scholar
- Ludascher B, Altintas I, Berkley C, Higgins D, Jaeger E, Jones M, Lee Edward A, Tao J, Zhao Y: Scientific workflow management and the Kepler system. Concurr Comp Pract Exper 2006, 18: 1039–1065. 10.1002/cpe.994View ArticleGoogle Scholar
- Marani M, Tommaso Z, Belluco E, Silvestri S, Maritan A: Non-neutral vegetation dynamics. PLoS One 2006,1(1):e78. 10.1371/journal.pone.0000078View ArticleGoogle Scholar
- Martin TE: Diversity and abundance of spring migratory birds using habitat islands on the Great Plains. Cooper Ornithol Soc 1980, 82: 430–439.Google Scholar
- McCuen RH, Knight Z, Cutter AG: Evaluation of the Nash-Sutcliffe Efficiency Index. Hydrol Eng 2006, 11: 597–602. 10.1061/(ASCE)1084-0699(2006)11:6(597)View ArticleGoogle Scholar
- Miao S: Rhizome growth and nutrient resorption: mechanisms underlying the replacement of two clonal species in Florida Everglades. Aquat Bot 2004, 78: 55–66. 10.1016/j.aquabot.2003.09.001View ArticleGoogle Scholar
- Miao SL, Sklar FH: Biomass and nutrient allocation of sawgrass and cattail along a nutrient gradient in the Florida Everglades. Wetlands Ecol Manage 1998, 5: 245–264.View ArticleGoogle Scholar
- Michalski F, Peres CA: Disturbance-mediated mammal persistence and abundance-area relationships in Amazonian forest fragments. Conserv Biol 2007, 21: 1626–1640.Google Scholar
- Muller S: Adaptive spatially-distributed water-quality modeling: an application to mechanistically simulate phosphorus conditions in the variable-density surface-waters of coastal Everglades wetlands. PhD Dissertation. University of Florida, Gainesville; 2010.Google Scholar
- Muneepeerakul R, Bertuzzo E, Lynch HJ, Fagan WF, Rinaldo A, Rodriguez-Iturbe I: Neutral metacommunity models predict fish disversity patterns in Mississippi-Missouri basin. Nature 2008, 453: 220–222. 10.1038/nature06813View ArticleGoogle Scholar
- Muñoz-Carpena R, Parsons JE, Gilliam JW: Modeling hydrology and sediment transport in vegetative filter strips. J Hydrol 1999, 214: 111–129. 10.1016/S0022-1694(98)00272-8View ArticleGoogle Scholar
- Newman S, Schutte J, Grace J, Rutchey K, Fontaine T, Reddy K, Pietrucha M: Factors influencing cattail abundance in the northern Everglades. Aquat Bot 1998, 60: 265–280. 10.1016/S0304-3770(97)00089-2View ArticleGoogle Scholar
- Odum HT, Odum EC, Brown MT: Wetlands management. In Environment and society in Florida. CRC Press, Boca Raton; 2000.Google Scholar
- Ott RL, Longnecker MT: A first course in statistical methods. Curt Hinrichs, Belmont, CA; 2004.Google Scholar
- Paradis E: Moran’s autocorrelation coefficient in comparative methods. 2010. . Accessed 7 August 2010 http://cran.r-project.org/web/packages/ape/vignettes/MoranI.pdf . Accessed 7 August 2010Google Scholar
- Perez-Ovilla O: Modeling runoff pollutant dynamics through vegetative filter strips: a flexible numerical approach. PhD Dissertation. University of Florida, Gainesville; 2010.Google Scholar
- Richardson CJ, King Ryan S, Vymazal J, Romanowicz Edwin A, Pahl James W: Macrophyte community responses in the Everglades with an emphasis on cattail (Typha domingensis) and sawgrass (Cladium jamaicense) interactions along a gradient of long-term nutrient additions, altered hydroperiod, and fire. Ecol Stud 2008, 201: 215–260. 10.1007/978-0-387-68923-4_9View ArticleGoogle Scholar
- Rivero RG, Grunwald S, Bruland GL: Incorporation of spectral data into multivariate geostatistical models to map soil phosphorus variability in a Florida wetland. Geoderma 2007, 140: 428–443. 10.1016/j.geoderma.2007.04.026View ArticleGoogle Scholar
- Rivero RG, Grunwald S, Osborne TZ, Reddy KR, Newman S: Characterization of the spatial distribution of soil properties in Water Conservation Area 2A, Everglades, Florida. Soil Sci 2007, 172: 149–166. 10.1097/01.ss.0000240550.52175.35View ArticleGoogle Scholar
- Rutchey K: Typha domingensis maps of WCA2A for the years 1991 and 1995. Personal communication. South Florida Water Management District, West Palm Beach; 2011.Google Scholar
- Rutchey K, Schall T, Sklar F: Development of vegetation maps for assessing Everglades restoration progress. Wetlands 2008,172(2):806–816.View ArticleGoogle Scholar
- SFWMD: Land cover land use 1995. 1995. . Accessed 11 November 2009 http://my.sfwmd.gov/gisapps/sfwmdxwebdc/dataview.asp?query=unq_id=297 . Accessed 11 November 2009Google Scholar
- SFWMD: Land cover land use 1999. 1999. . Accessed 11 November 2009 http://my.sfwmd.gov/gisapps/sfwmdxwebdc/dataview.asp?query=unq_id=1593 . Accessed 11 November 2009Google Scholar
- SFWMD: Documentation of the South Florida Water Management Model version 5.5. South Florida Water Management District, West Palm Beach, FL; 2005a.Google Scholar
- SFWMD: Regional Simulation Model (RSM) Hydrologic Simulation Engine (HSE) user’s manual. South Florida Water Management District, West Palm Beach, FL; 2005.Google Scholar
- SFWMD: Regional Simulation Model (RSM) theory manual. South Florida Water Management District, West Palm Beach, FL; 2005.Google Scholar
- SFWMD: RSM water quality user manual (draft). South Florida Water Management District, West Palm Beach, FL; 2008.Google Scholar
- SFWMD: RSMWQE theory manual (draft). South Florida Water Management District, West Palm Beach, FL; 2008.Google Scholar
- SFWMD: WCA2A HSE setup. South Florida Water Management District, West Palm Beach, FL; 2008.Google Scholar
- SFWMD: DBHYDRO. 2009. . Accessed 04 August 2010 http://my.sfwmd.gov/dbhydroplsql/show_dbkey_info.main_menu . Accessed 04 August 2010Google Scholar
- Stroustrup B: The C++ programming language. special edition. Addison-Wesley, Westford, MA; 2000.Google Scholar
- Tarboton KC, Irizarry-Ortiz MM, Loucks DP, Davis SM, Obeysekera JT: Habitat suitability indices for evaluating water management alternatives. South Florida Water Management District, West Palm Beach, FL; 2004.Google Scholar
- Urban NH, Davis SM, Aumen NG: Fluctuations in sawgrass and cattail densities in Everglades Water Conservation Area 2A under varying nutrient, hydrologic, and fire regimes. Aquat Bot 1993, 46: 203–223. 10.1016/0304-3770(93)90002-EView ArticleGoogle Scholar
- USACE, S.F.R.O: CERP: The plan in depth - part 1. 2010a. . Accessed 3 August 2010 http://www.evergladesplan.org/about/rest_plan_pt_01.aspx . Accessed 3 August 2010Google Scholar
- USACE, S.F.R.O: CERP: The plan in depth - part 2. 2010b. . Accessed 3 August 2010 http://www.evergladesplan.org/about/rest_plan_pt_02.aspx . Accessed 3 August 2010Google Scholar
- van der Valk AG, Rosburg TR: Seed bank composition along a phosphorus gradient in the northern Florida Everglades. Wetlands 1997,17(2):228–236. 10.1007/BF03161411View ArticleGoogle Scholar
- Walker WW, Kadlec RH: A model for simulating phosphorus concentrations in waters and soils downstream of Everglades stormwater treatment areas. Draft. US Department of the Interior Everglades National Park, Homestead, FL; 1996. http://publicfiles.dep.state.fl.us/DEAR/GoldAdministrativeRecord/Item%2027/018752.PDFGoogle Scholar
- Wang N: 2003 Vegetation map; dss hydrology input files. Personal communication. South Florida Water Management District, West Palm Beach, FL; 2009.Google Scholar
- Wang JD, Swain ED, Wolfert MA, Langevin CD, James DE, Telis PA: Application of FTLOADDS to simulate flow, salinity, and surface-water stage in the southern Everglades, Florida. Scientific Investigations Report 2007–2010. United States Geological Survey, Florida; 2007.Google Scholar
- Wetzel PR: Plant community parameter estimates and documentation for the Across Trophic Level System Simulation (ATLSS). East Tennessee State University, Johnson City; 2001.Google Scholar
- Wetzel PR: Nutrient and fire disturbance and model evaluation documentation for the Actoss Trophic level System Simulation (ATLSS). East Tennessee State University, Johnson City; 2003.Google Scholar
- Willard DA: SOFIA - FS-146–96. 2010. . Accessed 3 August 2010 http://sofia.usgs.gov/publications/fs/146–96/ . Accessed 3 August 2010Google Scholar
- Wu Y, Sklar FH, Rutchey K: Analysis and simulation of fragmentation patterns in the Everglades. Ecol Appl 1997,7(1):268–276. 10.1890/1051-0761(1997)007[0268:AASOFP]2.0.CO;2View ArticleGoogle Scholar
- Zajac ZB: Global sensitivity and uncertainty analysis of spatially distributed watershed models. PhD Dissertation. University of Florida, Gainesville; 2010.Google Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.