Open Access

Exclosures restored the density and root colonization of arbuscular mycorrhizal fungi in Tigray, Northern Ethiopia

Ecological Processes20176:33

https://doi.org/10.1186/s13717-017-0101-9

Received: 18 May 2017

Accepted: 29 August 2017

Published: 18 September 2017

Abstract

Introduction

Exclusion of grazing animals and tree plantations were among the methods used for the restoration of degraded lands in tropical semiarid areas. Exclosures can foster secondary forest succession by improving soil conditions and modifying microclimate for understory growth. This paper compared the arbuscular mycorrhizal fungi (AMF) spore density, root colonization of woody plants and soil chemical properties under exclosure with increasing age, and grazing land at different slope positions.

Methods

The study was conducted in northern Ethiopia from 12 exclosure sites paired each with adjacent grazing land in total from 24 sites with four treatments replicated three times. In the entire study, 216 plots were examined of which 108 were in exclosures and 108 in communal grazing lands. There were four age classes and three slope positions in each of the land uses. Composite soil and root samples were collected using nested plots measuring 100m2 from four sides of 1763 plants for spore enumeration and root colonization. Soils for chemical properties were collected from the four corners and center of 5 m × 5 m plots.

Results

All the 61 woody plant species that belong to 41 families were colonized by AMF. Spore density and root colonization were significantly higher in exclosures as compared to grazing land and increased with increasing ages of exclosures. Foot slope had significantly higher spore density and root colonization than middle and upper slopes. Soil chemical properties were significantly higher in the exclosure, oldest age of exclosure, and foot slope position (except P). AMF spore density and root colonization were significantly positively correlated with soil chemical properties.

Conclusions

Exclosures are helpful to restore the AMF spore density and root colonization of woody species and soil fertility.

Keywords

Spore density Root colonization Age of exclosure Slope position

Introduction

Exclosures are areas closed off from the interference of human and domestic animals with the goal of promoting natural regeneration of plants and reducing land degradation of formerly degraded communal grazing lands (Nedessa et al. 2005). They are usually established in steep, eroded, and degraded areas that have been used for grazing in the past (Descheemaeker et al. 2006a, b). In Ethiopia, the inception of exclosures dates back to the 1980s and coincided with the introduction of large-scale land rehabilitation and soil and water conservation program (Nedessa et al. 2005). The main objective of establishing exclosures on communal grazing lands is to improve the overall ecological conditions of the degraded areas so that they can provide better environmental and socioeconomic benefits to the local communities. Several case studies have reported beneficial effects of exclosures: natural vegetation has regenerated, runoff, and sheet and rill erosion are reduced, the land is stabilized, water availability has increased, and soil physical and chemical properties are improved (Girmay et al. 2009; Mekuria et al. 2009; Mekuria et al. 2007; Yami et al. 2007; Descheemaeker et al. 2006a, b). These positive consequences are also noticed and appreciated by the local communities (Mekuria et al. 2009).

The impact of grazing on soil properties depends on grazing management, the non-grazed control site provided higher soil pH, available P, and Mg compared with moderately grazed site of 33 years old (Ajorlo et al. 2011). Available phosphorus (P), total nitrogen (N), calcium (Ca), magnesium (Mg), and potassium (K) decreased after 1.5 years of open grazing compared with an ungrazed control in a tropical pasture (Ajorlo et al. 2011). In addition, open grazing resulted in lower water infiltration (Hiernaux et al. 1999) and higher soil loss (Tadesse and Penden 2002) compared with moderately grazed sites. Exclosures with 12 years old enhance the total plant cover, dry matter yield, species richness, and contents of soil organic matter, total nitrogen, and water infiltration rate compared with continually grazed area (Jeddi and Chaieb 2010). Exclusion of livestock grazing for 20 years significantly increased aboveground and belowground biomass and species richness for five different communities compared with non exclosed sites in Loess Plateau, northwest China (Cheng et al. 2011). The exclosures had twice more plant species richness and diversity value as compared with communal grazing lands after 22 years of exclosure establishment in the central and northern highlands of Ethiopia (Mengistu et al. 2005). There were increases in the number of woody species richness after 8 years of exclosure establishment (Birhane et al. 2006), 1.1% increase in soil organic matter, 0.1% in total N, 1.8 mg kg−1 in available P increase after 10 years of exclosure establishment (Mekuria et al. 2007). A considerable decrease in soil loss was reported after the establishment of exclosures on communal grazing lands (Girmay et al. 2009; Mekuria et al. 2009; Descheemaeker et al. 2006a, b). Exclosures have been reported to positively contribute to restore soil biological properties such as arbuscular mycorrhizal fungi (Birhane et al. 2010).

Arbuscular mycorrhizal fungi (AMF) colonize about 80% of plants (Wang and Zao 2008) and they are regular component of rhizosphere micro-flora in natural ecosystem (Sharma et al. 2009). AMF form obligate symbiotic association with many tropical plants (Manoharachary et al. 2005). The function of AMF in exclusion process and knowledge of AMF diversity in a specific area is essencial to utilize AMF in any application (Wang and Zao 2008). The very few conditions where infective AMF are low in density and diversity is when the soil erodes, is disturbed, and is devoid of vegetation cover (Asmelash et al. 2016). The presence of enough AMF propagules in a restored site facilitates the establishment of plant communities through increased availability of nutrients and moisture to plant roots (Asmelash et al. 2016). Though exclosures positively contributed to land rehabilitation, there are limited studies on the role of exclosures to restore arbuscular mycorrhizal fungi. There is a limited study that attempted to understand the role of exclosure in the restoration of AMF with increasing age of exclosures at different slope positions during the rainy season. The slope position significantly affects the density of spores found in a given landscape. The spore density was found to be higher from exclosures of middle slope position in the dry season (Birhane et al. 2017). We investigated the spore density in the soils and woody plant root colonization of AMF from paired exclosure and adjacent grazing land with increasing age of exclosures at the foot, middle, and upper slope hillsides. The correlation between the availability of soil nutrients with AMF spore density and root colonization was assessed. We aimed to answer the following questions: What is the dynamics of AMF with increasing age exclosures at different slope positions? Does the availability of nutrients correlated with AMF spore density and root colonization? And does the increase in the age of exclosures increased the availability of nutrients at different slope positions?

Methods

Study area

The study was conducted in the highlands of Tigray region, Northern Ethiopia in 12 sites representing four zones and four districts (Table 1). All sites have tropical semi-arid climate. The altitude of the study sites ranged from 2232 to 2937 m.a.s.l. The rainy season usually occurs between June and September (Fig. 1), and the growing season varies between 90 and 120 days. The highest rainfall is in July and August which ranges between 162 and 228 mm. The temperature of the study sites ranges from 19 to 30 °C, the maximum is recorded in the months of May and June (Fig. 1).
Table 1

Specific study site age, altitude and geographic location

Specific site

Zone

District

Age (year)

Altitude (m)

Geographic location

Adihintaweinai

East

Atsbi wonberta

< 10

2201–2312

39° 38′ 60″–39° 50′ 52 East

13° 12′ 75–14° 4′ 41 North

Halla

Southeast

Degua Tembien

< 10

2232–2937

38° 30′ 17–38° 40′ 57 East

13° 54′ 3–14° 20′ 30 North

Melgim

East

Atsbi wonberta

< 10

2264–2343

39° 38′ 60–39° 50′ 52 East

13° 12′ 75–14° 4′ 41 North

Endagebriel

East

Atsbi wonberta

10–20

2248–2351

39° 38′ 60–39° 50′ 52 East

13° 12′ 75–14° 4′ 41 North

Gurzoemni

Central

Tahtay Maichew

10–20

2244–2322

38° 30′ 17–38° 40′ 57 East

13° 54′ 3–14° 20′ 30 North

Mezewle

East

Atsbi wonberta

10–20

2325–2411

39° 38′ 60–39° 50′ 52 East

13° 54′ 3–14° 20′ 30 North

Adikolakul

Southeast

Degua Tembien

20–30

2180–2214

38° 30′ 17–38° 40′ 57 East

13° 54′ 3–14° 20′ 30 North

Suhulkoma

East

Atsbi wonberta

20–30

2295–2347

39° 38′ 60–39° 50′ 52 East

13° 12′ 75–14° 4′ 41 North

Wereriba

Southeast

Doguetembien

20–30

2200–2358

13° 16′ 23–13,047′ 44″ East

39° 3′ 17″–39,024′ 48″ North

Gratselim

Southeast

Degua Tembien

30–40

2369–2458

38° 30′ 17–38° 40′ 57 East

13° 54′ 3–14° 20′ 30 North

Kerenadidemsash

South

Endamekoni

30–40

2314–2419

39° 16′ 52–39° 35′ 31 East

12° 38′ 4–12° 51′ 39 North

Maibiati

Southeast

Degua Tembien

30–40

2358–2429

38° 30′ 17–38° 40′ 57 East

13° 54′ 3–14° 20′ 30 North

Fig. 1

Monthly average rainfall (RF), maximum temperature (MaxT) and minimum temperature (MinT) of the study area from 2002 to 2014 (EMA 2015)

Luvisols (Alfisols), Regosols (Entisols), Cambisols (Inceptisols), and Calcisols (Aridisols) were major soil groups in the study area (WRB 2006). The 24 study sites were dominanted by Luvisols (Alfisols) and Cambisols (Inceptisols). Acacia etbaica, Acacia seyal (Del.), Becium grandiflorum (Lam.) Pichi-Serm., Euclea racemosa subsp. schimperi (A. DC.) Dandy, and Maytenus arbutifolia (Hochst. ex. A. Rich) Wilczek were the woody vegetation species common in exclosures and adjacent grazing lands (Mekuria 2010).

Mixed farming system that integrates crop and livestock was the main means of livelihood. The major land uses were cultivated land, forest land, exclosure, and communal grazing land (Mekuria 2010). The main crops cultivated were Teff (Eragrostis teff (Zucc.) Trotter), Bread wheat (Triticum aestivum), Maize (Zea mays L.), Sorghum (Sorghum bicolor), Barley (Hordeum vulgare), and Faba bean (Vicia faba). The main animal which graze the communal grazing lands were cattle, goats, sheep, donkeys, camel, mule, and horse.

Experimental design

The age of restoration, slope position, and land use effect on AMF spore density, woody plant root colonization, and soil chemical properties were studied from 12 exclosure sites paired each with adjacent grazing land during the dry season. The distance between the exclosure and the adjacent grazing land was in the range of 50 to 100 m. There were four age classes and three slope positions in each of the land uses. The first age group had less than 10 years old exclosure with triplicate sites (Halla, Meligim, and Adihantaweynay), the second age group was 10 to 20 years old exclosure with triplicate sites (Gurzoemni, Mezewle, and Endagebriel), and the third age group had 20 to 30 years old exclosure with triplicates sites (Addikolakul, Wereriba, and Shul-koma). The fourth group had greater than 30 years old exclosure with triplicate sites (Maybe’ati, Kerenadidemsash, and Gratselim). The assumption were prior to establishment, exclosures and the adjacent grazing lands had similar biophysical, socioeconomic, and management conditions as the exclosures were established on the same communal grazing lands which were used for livestock grazing (Mekuria 2010). The experiment was composed of 12 experimental units with four treatments replicated three times. Site had two levels (closed and open), age with four levels (< 10, 10–20, 20–30, and > 30 years old) with three slope positions (upper, middle, and foot slope). In the entire study, 216 plots (12 × 3 small plots × 3 slope positions × 2 pair exclosure and adjacent grazing land) were examined of which 108 were in exclosures and 108 in communal grazing lands. The small plots used to collect soil samples. In each exclosure and grazing land randomly established three transects spaced at a minimum distance of 75 m (Mekuria 2010).

The number of transects were based on vegetation density, spatial heterogeneity of vegetation, and area of the site. The area of the selected exclosures ranged from 7 to 85 ha, whereas that of the adjacent communal grazing lands ranged from 2 to 38 ha. To avoid edge effects, the first transect was laid 30–50 m inside the exclosures and grazing lands. Transects were parallel to each other and to the topography of the landscape. In each transect, three slope positions (upper slope (US), mid slope (MS), and foot slope (FS)) were delineated and in each landscape position, a sampling plot measuring 10 m × 10 m was established. In each plot, 5 m × 5 m subplots for soil physical and chemical analysis were developed. The US position is the uppermost portion of each study site, and it can receive little or no overland flow but may contribute runoff to down slope areas. The MS position receives overland flow from the upper slope and contributes runoff to the FS. The FS represents the lowest part of each study site and receives overland flow from both mid and upper slopes.

Plant and soil sampling methods

The plant roots and soils were sampled during the dry season from October 2013 to January 2014 for a total of 4 months from exclosures and adjacent surrounding grazing lands. All woody plants were sampled from the 10 m × 10 m quadrates (100 m2) for trees and shrubs from both land uses. Woody plants were replicated three times in each plot. Composite soil and root samples were collected for spore density and root colonization from a total of 1763 samples from 4 sides of each plant found in each plot from the exclosures and adjacent grazing lands. The identity of the plant was identified at the field and verified using reference books such as Bekele (2007), Inga et al. (2003), Sue et al. (1995, 2000), and Inga and Sue (1989).

Soil samples were collected from 5 m × 5 m subplots nested with in the 10 m × 10 m at the center of the main plots. A total of 216 soil samples were collected. The soil samples for one plot was taken from the four corners and the center of a square plot at 0–50 cm soil depths following an “X” pattern of the main plot to form one composite sample in order to determine texture, soil organic carbon, pH, EC, N, K, and P in exclosures and adjacent grazing lands. The five soil samples measured 300 g each were mixed and form 1500 g in total and make single composite sample to represent the sample plot. From the composite sample, 1 kg soil was taken and was put into plastic bags, secured, labeled and brought to the soil laboratory. Soil samples were analyzed for pH and electrical conductivity on 1:2.5, soil: water suspension method. Organic matter was analyzed using the Walkley-Black method (Van Ranset et al. 1999), total nitrogen content by the Kjeldahl method (Bremmery and Mulvaney 1982). Available potassium and texture were analyzed with flame spectrophotometer and hydrometer method (Gee and Bauder 1982). Available P determined using the Olson method (Olsen and Sommers 1982).

AMF root length colonization and spore density sampling

Root samples of all plants were collected by excavating soil using a handheld hoe starting from the plant’s trunk base in four directions of the plant and working out towards to get fine roots within 3–5 m radius. Forty-gram root samples from all four directions were collected from 0 to 30 cm depth, mixed and then 10 g fine roots (diameter < 2 mm) were taken from the composite root samples. The collected fine roots were put into beaker and carefully washed with tap water until the soil was removed and became free of any soil particles and put into plastic jar and filled with 97% ethanol to preserve the roots until they were ready for processing. Soil samples were taken at 50 cm depth using auger (Chanie and Assefa 2013) in four directions of each plant and gave 300 g soil sample, in total 1200 g soil sample were mixed to make one composite soil sample. For each plant, 1 kg composite soil samples was taken from the 1200 g of soil and put in a sealed plastic bag. The sampled roots and soils were transported to Mekelle University laboratory for estimating root colonization and spore extraction.

AMF spores were separated from the soil by the wet sieving and 50% sucrose centrifugation method (Brundrett et al. 1996). For spore extraction, the soils first air-dried and pass through 0.75 mm sieve and weighed using sensitive balance to get a subsample of 25 g. The weighed dry soil was wet with enough amount of water for at least 30 min. The wet soil was stirred for 15 to 30 min and then decanted through a series of sieves by putting in ascending order with the smallest sieve size (50 μm) at the bottom, followed by 100, 300, and 750 μm sieve size at the upper most. The 750 μm sieve size used to remove unnecessary materials. The different sieve sizes were given codes as 50 μm = A, 100 μm = B, and 300 μm = C, to designate the spore sizes captured by each sieve sizes. Spores were counted from 25 g soil using dissecting microscope with × 100 magnification.

AMF spores were grouped into genera using their morphological characteristics such as spore size, shape, color, wall structure, and hyphal attachment (simple, swollen, or bulbous). Permanent slides were prepared for each different spore morphotype with polyvinyl alcohol plus Melzer’s solution. The PVLG mountant was prepared using polyvinyl alcohol 8.33 g (polyvinyl alcohol should be used and can be dissolved in water by heating at 90 °C overnight), distilled water 50 ml, lactic acid 50 ml, and glycerin 5 ml) and prepared Melzer’s reagent (1.5 g iodine, 5 g potassium iodide, and 100 ml distilled water). The Melzer’s reagent then mixed 1:1 (v/v) with PVLG (Brundrett et al. 1996). Small droplets of the mountant was added using pipet (1:1 PVLG and Melzer’s reagent) on to the microscope slide then the spores put on it using paint brush and pressed with coverslip to easily see and identify the internal structures of the spores. The genera of each spore were identified using a compound microscope with × 400 magnification based on descriptions in Brundrett et al. (1996) and information from the INVAM website (http://www.invam.caf.wvu.edu).

The procedure in Brundrett et al. (1996) was used to determine root colonization. To identify root colonization, the roots were washed to remove ethanol, cut into 1 cm length, and insert into the heat resistant bottle containing 10% KOH solution then autoclaved for 20 min at 120 °C. The roots were washed to remove KOH and put into 10% H2O2 for further bleaching and clearing for about 15 min. Cleared roots were captured on a fine sieve and rinsed with water before transferring them into the HCl solution. Roots were acidified with 3% HCl for about 30 min and stained in trypan blue (0.05% in 5:1:1 lactic acid: glycerol: distilled water) over night. The stained roots were washed and added to 50% glycerol for destaining and preserved until further processing. Then roots were prepared on slide lengthwise by selecting 20 subsample roots. The proportional root colonization by AMF was estimated using the magnified intersection method with hair line graticule inserted into an eyepiece acted as the line of intersection with each root at 400 magnification power under the compound microscope. Percentage of root length colonization (RLC %) was calculated from 100 or more intersections for each root sample. At each intersection, there were six possible mutually exclusive outcomes (Brundrett et al. 1994).

Statistical analyses

The differences in spore density, root colonization, and soil parameters between an exclosure and its adjacent communal grazing land at different age groups and landscape position were assessed using two- and three-way ANOVA with Tukey HSD test after checking normality test. Pearson correlation test was used to conduct relationships between spore density and root colonization and soil parameters. Statistical package for social sciences (SPSS) version 20 was used to analyze spore density and root colonization of AMF and the soil.

Results and discussion

AMF spore density and root colonization between exclosures and adjacent grazing lands

Six AMF genera were recovered and identified in exclosures (Table 2). Glomus was the dominant genus found in all soils followed by Acaulospora, Gigaspora, Scutellospora, Entrophospora, and Sclerocystis. Glomus, Acaulospora, and Gigaspora were found in all the 12 sites followed by Scutellospora (11), Entrophospora (9), and Sclerocystis (1). This finding was similar to Vyas and Gupta (2014) where they found similar dominance order of genera. Exclosures had significantly higher spore density (4385 spores 100 g−1 dry soil and 6 genera) than grazing land (176 spores 100 g−1 dry soil and 5 genera). The genus Sclerocystis was retrieved from exclosure but not in the samples from grazing land.
Table 2

Spore density and spore genera in exclosures and grazing land (100 g−1 dry soil)

LU

Gigaspora

Scutellospora

Glomus

Sclerocystis

Acaulospora

Entrophospora

Sum

EX

206.3 ± 5.5a

105.2 ± 3.3a

2468.2 ± 42.2a

0.013 ± .009a

1582.2 ± 30.8a

23.2 ± 1.3a

4385.3 ± 80.4a

GL

2.3 ± 8.5b

0.271 ± 5.1b

110.2 ± 64.3b

0.000 ± .013a

63.3 ± 47.0b

0.068 ± 1.9b

176.3 ± 122.5b

Means followed by the same letter in the same column are not significantly different at P < 0.05 level, mean ± SEM

GL grazing land, EX exclosure, LU land use types

Exclosures had higher spore density mainly dominated by the genus Glomus in Northern Ethiopia (Birhane et al. 2010). The low spore density in grazing land could be due to high soil disturbance, changes in land use and management, and changes in plant community composition (Birhane et al. 2017; Santos-González et al. 2007).

The spore density recovered in this study was higher than the total number of spores recovered from forest and grazing land (Chris 2010). Glomus was the most dominant genus in exclosure and in grazing land. Glomus is a predominantly genus found to be distributed in soils all over the world (Minal and Anil 2012; Chris 2010) and is the dominant genus in disturbed soils (Tanvir et al. 2011; Burni et al. 2009; Sharma et al. 2009; Wang and Zao 2008; Mridha and Dhar 2007; Panwar and Tarafdar 2006; Burni and Illahi 2004). The predominance of Glomus species under varying soil conditions might be due to its wide adaptability to the varied soil conditions and survive in acidic as well as in alkaline soils (Tanvir et al. 2011; Pande and Tarafdar 2004).

Spore density in undisturbed natural environment is higher than disturbed sites (Chanie and Assefa 2013; Muleta et al. 2008). AMF plays a role in the formation of stable soil aggregates, building up a macroporous structure of soil that allows penetration of water and air and prevents erosion (Rillig 2004; Borie et al. 2008; Aggarwal et al. 2011); therefore, the application of AMF is of interest for the reclamation and revegetation of degraded lands (Miller and Jastrow 1992).

The influence of grazing on soil nutrient availability and host plant productivity (Frank and McNaughton 1993) may cause variable effects on AMF community composition and structure (Bai et al. 2013; Eom et al. 2001). Grazing intensity might change the level of mycorrhizal infection in a community by altering the plant composition and is an important factor in regulating the cycling of nutrients in undisturbed ecosystems (Abbott and Robson 1991). Grazing can influence the dynamics of nutrient exchange between host plants and AMF (Gehring and Whitham 2002). Herbivore grazing can alter leaf photosynthetic rates (McNaughton 1979), the above ground production (Frank and McNaughton 1993) and the C allocation below ground (Frank et al. 2002). The allocation of AMF morphological structures can either increase or decrease depending on the timing and severity of herbivores (Gange 2007).

Land use significantly affected AMF root colonization (Table 3). AMF root colonization was significantly higher in exclosures compared to the adjacent grazing land (P < 0.05). Hyphal colonization was the highest followed by MHC and AC while the least was VC. The grazing land showed low root colonization, and the higher colonization was for hyphae followed by MHC, AC, and VC, respectively.
Table 3

Root length colonization (%) of AMF between exclosures and open grazing lands

Land use types

HC

MHC

AC

VC

Exclosure

78.4 ± 0.4a

70.1 ± 0.5a

50.3 ± 0.5a

38.1 ± 0.5a

Grazing land

55.7 ± 0.6b

47.5 ± 0.8b

35.5 ± 0.8b

23.4 ± 0.8b

Means in the same column followed by same letter do not differ significantly at P < 0.05, mean ± SEM

HC hyphal colonization, MHC mycorrhizal colonization, AC arbuscular colonization, VC vesicular colonization

AMF spore density and root colonization with increasing age of exclosures and slope position

Spore density increased significantly as the age of exclosure increased (P < 0.05). An exclosure with greater than 30 age class counted higher spore density followed by age class between 20 and 30, age class between 10 and 20, and age class less than 10, respectively (Table 4). AMF spore density ranged from 25 to 2550 in 100 g−1 of dry soil with increasing stability of ecosystem (Zhao et al. 2003). High AMF diversity was found in undisturbed and old woodlands and forests (Don-Rodrigue et al. 2013; Mathimaran et al. 2007; Muthukumar and Udaiyan 2000; Wilson et al. 1992). Glomus, Acaulospora, and Gigaspora were highly significantly different in all age classes while Scutellospora and Entrophospora significantly differed only among age classes greater than 30, age class from 20 to 30, and 10–20 (Table 4). Except Sclerocystis and Entrophospora, all the genera were found in all age classes. The dominant genera at all stages of wheat crop development were Acaulospora, Glomus, and Scutellospora (Arpita et al. 2012; Schalamuk et al. 2006) and the dominant genera in tropical rainforest were Acaulospora and Glomus (Zhao et al. 2003). The increased trend in spore density with increased age of exclosures is related to the low disturbance and high organic matter in old age exclosure (Burni and Ilahi 2004; Anwar and Jalaluddin 1991). High organic matter increases the water holding capacity of soil which may enhance the sporulation of AMF.
Table 4

AMF spore density and spore genera in four age classes of exclosures (100 g−1 of dry soil)

Age (year)

Gigaspora

Scutellospora

Glomus

Sclerocystis

Acaulospora

Entrophospora

Sum

< 10

6.1 ± 10.6d

2.9 ± 6.3c

326.2 ± 91.7d

0.000 ± 0.01a

226.6 ± 64.2d

0.000 ± 2.5c

562.0 ± 169.3d

10–20

39.7 ± 8.4c

13.3 ± 5.0c

1135.9 ± 72.6c

0.000 ± 0.01a

603.4 ± 50.8c

2.5 ± 2.0c

1794.9 ± 134.1c

20–30

162.7 ± 8.5b

75.6 ± 5.1b

2002.2 ± 74.1b

0.000 ± 0.01a

1259.3 ± 51.8b

13.6 ± 2.0b

3513.6 ± 136.7b

> 30

300.8 ± 7.9a

165.2 ± 4.7a

2903.5 ± 68.6a

0.030 ± 0.01a

1976.0 ± 48.0a

39.9 ± 1.9a

5385.6 ± 126.6a

Means followed by the same letter in the same column are not significantly different at P < 0.05 levels, mean ± SEM

All AMF genera showed significant difference between slope positions except for Sclerocystis (Table 5). The foot and middle slope had six genera whereas the upper slope had five genera (Table 5). The highest spore density was recorded at the foot slopes followed by the middle and upper slopes. The interactions between age × land use, age × elevation, land use × elevation, and age × land use × elevation also showed significant difference in spore density (P = 0.000). This high spore density and genera richness increment from upper to down slope might be due to natural forces such as flood and wind. AM fungal spores rely on some forces that can move soil, such as strong winds, water, gophers, and worms, to migrate to a new location (David and Jeff 2010). Removal of surface soil layers mainly by water erosion decreased markedly both the number of propagules of AMF and the extent of mycorrhiza formation (Habte 1989; Powell 1981) thereby increase spore density and genera diversity down slope gradient.
Table 5

AMF spore density (100 g−1 of dry soil) across foot, middle, and upper slope position

Slope

Gigaspora

Scutellospora

Glomus

Sclerocystis

Acaulospora

Entrophospora

Sum

Foot

192.3 ± 8.2a

109.2 ± 4.8a

2578.0 ± 66.0a

0.006 ± 0.01a

1747.6 ± 45.6a

22.8 ± 1.8a

4650.1 ± 122.9a

Middle

133.8 ± 9.0b

60.3 ± 5.2b

1491.4 ± 72.1b

0.021 ± 0.01a

947.4 ± 49.9b

14.2 ± 1.9b

2647.3 ± 134.3b

Upper

96.8 ± 9.2c

42.8 ± 5.4c

1006.4 ± 4.1c

0.000 ± 0.01a

527.1 ± 51.2c

10.1 ± 2.0b

1683.4 ± 137.9b

Means followed by the same letter in the same column are not significantly different at P < 0.05 level, mean ± SEM

All the age groups in both land uses exhibited all structures of AMF. The root colonization showed significant difference among the four age classes (Table 6). There was higher colonization in % HC, % MHC, % AC, and % VC with increasing age from < 10 years of age to 10–20 years of age followed by 20–30 years of age, and the highest colonization was recorded in the age group > 30 years old. The root colonization of Juniperus procera ranged from 59 to 79% and increased with the age of the tree (Amal and Hasnah 2012). Natural environment increased spore density and root colonization, indicating stability of ecosystems (Megan and Kirkegaard 2012).
Table 6

Root colonization (%) of AMF structures at four age groups of exclosure

Age (year)

HC

MHC

AC

VC

< 10

57.0 ± 0.9d

39.9 ± 1.0d

23.7 ± 0.9d

10.3 ± 0.9d

10–20

65.8 ± 0.7c

55.5 ± 0.8c

37.3 ± 0.7c

23.3 ± 0.7c

20–30

74.0 ± 0.7b

68.9 ± 0.8b

51.7 ± 0.7b

39.7 ± 0.7b

> 30

82.7 ± 0.6a

78.4 ± 0.7a

60.7 ± 0.6a

50.7 ± 0.6a

Means in the same column followed by same letter do not differ significantly at P < 0.05, mean ± SEM

HC hyphal colonization, MHC mycorrhizal hyphal colonization, AC arbuscular colonization, VC vesicular colonization

Plant roots at the foot slope showed higher colonization compared to the plants at the middle and upper slope positions. Mycorrhizal hyphal colonization was significantly different between the foot slope and the other two slope position (P < 0.05). The interaction between age × land use and land use × elevation were sources of variation (P = 0.000) in root length colonization. Soil disturbance, degradation, and erosion can reduce levels of AMF propagules (Birhane et al. 2010; Carpenter et al. 2001; Brundrett et al. 1996). Vesicular, arbuscular, and hyphal colonization in soils of soybean plant root found colonized with AMF in 133 samples out of 167 samples examined at different slope positions (Bansode et al. 2014). Current distribution and density of AMF are the result of contemporary ecological processes that are under control of several factors such as altitude, soil chemical properties, soil disturbance, and above-ground vegetation (Sturmer and Siqueira 2011; Yang et al. 2010; Shukla et al. 2009).

AMF in different woody plant species and their families

All rhizosphere soil samples under each plant species generally displayed higher spore density as compared to other studies and were shown significant variation in spore density between plant species and their family (Table 7), though the grazing intensity varies with species. The highest spore density was found under Ficus vasta, Cordia africana, Ekeberrgia capensis, and Justicia schimperiana while Aloe vera, Opuntia ficus-indica, and Rumex nervosus exhibited the lowest spore density. Families of Acanthaceae, Boraginaceae, Meliaceae, and Moraceae had significantly higher spore density 100 g−1 dry soil while lowest spore counts were found in Aloeaceae, Cactaceae, and Polygonaceae rhizosphere soils. Low AMF spore count was reported by Muleta et al. (2008) for coffee and in a dry savannah wood land ecosystem under Acacia polyacantha rhizosphere soil (Yonas 2005).
Table 7

AMF spore density (100 g−1 dry soil) and root colonization (%) of plant species and their families

AMF between plant species

AMF between plant families

Plant species

Spore abundance

Root length colonization

Plant families

Spore abundance

Root colonization

Abutilon longicuspe

5173.6 ± 194.9bcde

81.7 ± 5.5abcdefg

Acanthaceae

12,463.0 ± 396.2a

80.0 ± 15.9abcdef

Acacia decurrens

2254.2 ± 619.4cdef

68.6 ± 3.1fghij

Aloeaceae

312.4 ± 608.9e

57.5 ± 3.1ghi

Acacia etbaica

2768.3 ± 287.5bcdef

73.0 ± 1.4cdefghij

Anacardiaceae

5505.4 ± 586.7bcd

92.1 ± 3.0abc

Acacia lahai

3224.2 ± 194.9bcdef

83.3 ± 5.5abcdefg

Apocynaceae

6018.8 ± 422.5bc

83.9 ± 2.1abcde

Acacia saligna

3065.0 ± 406.6bcdef

72.2 ± 2.0cdefghij

Asclepiadaceae

2365.5 ± 189.3cde

85.5 ± 11.2abcd

Acokanthera schimpri

6868.2 ± 632.1ab

90.5 ± 3.1abcde

Berberidaceae

2023.0 ± 189.3de

80.0 ± 11.2abcdef

Acacia seyal

3893.2 ± 272.6bcdef

78.2 ± 1.3abcdefgh

Boraginaceae

12,982.0 ± 189.3a

100.0 ± 11.2a

Aloe vera

312.4 ± 607.3f

57.5 ± 3.01hij

Buddleiaceae

6031.0 ± 1097.7bc

86.3 ± 5.6abcd

Berberis holstii

2023.0 ± 2181.6def

80.0 ± 10.9abcdefg

Cactaceae

173.7 ± 677.5e

56.9 ± 3.4hi

Becium grandiflorum

2344.2 ± 252.0cdef

65.8 ± 1.2fghij

Capparidaceae

2098.6 ± 896.2de

62.4 ± 4.5fghi

Buddleja polystachya

5144.0 ± 979.3bcde

81.2 ± 4.9abcdefg

Celastraceae

2849.4 ± 240.2cde

68.4 ± 1.2defghi

Calpurinia aurea

3318.8 ± 360.0bcdef

72.7 ± 1.8cdefghij

Combretaceae

5038.9 ± 548.8bcd

72.8 ± 2.8cdefghi

Cadaba farinose

2098.6 ± 894.0def

62.4 ± 4.5ghij

Cupressaceae

2331.1 ± 238.8cde

76.4 ± 1.2bcdefgh

Carissa spinarum

5339.3 ± 565.4bcde

78.6 ± 2.8abcdefgh

Ebenaceae

1769.8 ± 211.7de

62.2 ± 1.0fghi

Clutia lanceolata

2024.0 ± 3085.3def

52.0 ± 15.5j

Ericaceae

5330.5 ± 189.3bcd

73.50 ± 11.2bcdefghi

Combretum aculeatum

5038.9 ± 547.4bcde

72.8 ± 2.7cdefghij

Euphorbiaceae

4839.5 ± 457.7bcd

77.0 ± 2.3abcdefg

Cordia africana

12,982.0 ± 2181.6a

100.0 ± 10.9a

Fabaceae

3185.5 ± 148.6bcde

73.3 ± .7bcdefghi

Croton macrostachyus

5729.4 ± 710.5bcde

83.2 ± 3.5abcdefg

Flacourtiaceae

6738.6 ± 1792.5ab

96.3 ± 9.1a

Cupressus lusitanica

2433.6 ± 710.5cdef

86.7 ± 3.5abcdef

Lamiaceae

2380.7 ± 251.0cde

65.7 ± 1.2efghi

Diospyros abyssinica

4893.0 ± 2181.6bcde

98.0 ± 10.9a

Loganiaceae

5144.0 ± 981.8bcd

81.2 ± 5.0abcdef

Diplostigma canescens

2365.5 ± 2181.6cdef

85.5 ± 10.9abcdef

Loranthaceae

3533.0 ± 189.3bcde

85.5 ± 11.2abcd

Dovyalis abyssinica

6738.6 ± 1788.1ab

96.3 ± 9.0a

Malvaceae

5173.6 ± 1097.7bcd

81.7 ± 5.6abcdef

Dodonaea angustifolia

3857.7 ± 285.1bcdef

76.2 ± 1.4abcdefghi

Meliaceae

10,498.0 ± 1173.5a

80.8 ± 6.0abcdef

Ekeberrgia capensis

10,498.0 ± 170.5a

80.8 ± 5.9abcdefg

Moraceae

11,797.0 ± 189.3a

88.5 ± 11.2abcd

Erica arborea

5330.5 ± 2181.6bcde

73.5 ± 10.9cdefghij

Myrtaceae

2599.9 ± 275.5cde

69.2 ± 1.4defghi

Euphorbia abyssinica

4213.3 ± 596.0bcdef

72.6 ± 3.0cdefghij

Oleaceae

6890.6 ± 411.2ab

92.9 ± 2.1ab

Eucalyptus camaldulensis

2809.6 ± 319.4bcdef

69.6 ± 1.6efghij

Oliniaceae

3494.5 ± 861.1bcde

70.9 ± 4.4defghi

Eucalyptus globules

2002.6 ± 539.16def

68.1 ± 2.7fghij

Polygonaceae

264.0 ± 694.2e

55.1 ± 3.5i

Euclea racemosa

1769.8 ± 211.2ef

62.2 ± 1.0ghij

Proteaceae

2789.0 ± 2189.3cde

87.0 ± 11.2abcd

Ficus vasta

11,797.0 ± 2181.6a

88.5 ± 10.9abcdef

Rosaceae

7163.5 ± 2189.3ab

100.0 ± 11.2a

Gravillea robusta

2789.0 ± 2181.6bcdef

87.0 ± 10.9abcdef

Rubiaceae

3013.0 ± 2189.3bcde

76.5 ± 11.2bcdefgh

Juniperus procera

2318.1 ± 252.8cdef

75.0 ± 1.2bcdefghij

Sapindaceae

3857.7 ± 285.8bcde

76.2 ± 1.4bcdefgh

Justicia schimperiana

12,463.0 ± 3085.3a

80.0 ± 15.5abcdefg

   

Maytenus arbutifolia

2918.5 ± 250.3bcdef

68.6 ± 1.2fghij

   

Maytenus senegalensis

2094.7 ± 827.7def

65.8 ± 4.1fghij

   

Nuxia congesta

6031.0 ± 194.9bcd

86.3 ± 5.5abcdef

   

Olea europaea

6890.6 ± 410.2ab

92.9 ± 2.0abc

   

Olinia rochetiana

3494.5 ± 858.9bcdef

70.9 ± 4.3defghij

   

Opuntia ficus indica

173.7 ± 675.8f

56.9 ± 3.4ij

   

Osyris quadripartite

3533.0 ± 2181.6bcdef

85.5 ± 10.9abcdef

   

Otostegia integrifolia

2213.2 ± 185.0def

58.0 ± 7.0hij

   

Psydrax schimperiana

3013.0 ± 2181.6bcdef

76.50 ± 10.9abcdefghi

   

Rhus glutinosa

5137.0 ± 692.5bcde

91.1 ± 3.5abcd

   

Rhus retinorrhoea

6426.5 ± 194.9abc

94.8 ± 5.56ab

   

Rosa abyssinica

7163.5 ± 2181.6ab

100.0 ± 10.9a

   

Rumex nervosus

264.4 ± 751.1f

54.9 ± 3.7j

   

Senna singueana

2307.7 ± 607.3cdef

56.5 ± 3.0ij

   

Means in the same column followed by same letter do not differ significantly at P < 0.05 for spore abundance and root colonization of plants in highlands of Tigray. Mean ± SEM

Glomus was found in all the 32 plant families and 47 plant species followed by Acaulospora, Gigaspora, Scutellospora, Entrophospora, and the lowest found in Sclerocystis, respectively (Additional file 1: Table S1 and S2). Glomus is the most common AMF genus distributed globally and dominant in the tropical areas (Chaurasia 2000). Wide occurrence of Glomus in the present study suggested that the genus Glomus has wide ecological amplitude that is responsible for its adaptability and survival in different habitats and vegetation composition. The dominance of Glomus species has been reported from tree species in Ethiopia (Birhane et al. 2010; Wubet et al. 2003, 2009; Muleta et al. 2008). The variation in spore density between plants species may be due to differences in host trees which can be attributed to the type of AMF species associated with their respective hosts (Cardoso et al. 2003; Pringle and Bever 2002).

Typical structures like hyphae, arbuscules, and vesicles were observed in all tree species (Additional file 1: Table S3 and S4). This study was in agreement with Allen et al. (1998) and found all plant species to be colonized by AMF. There were a significant difference (P = 0.000) in root length colonization between plant species and their families (Table 7). Cordia africana, Diospyros abyssinica, Dovyalis abyssinica, and Rosa abyssinica showed significant difference while Clutia lanceolata and Rumex netvosus showed the lowest root length colonization. Boraginaceae, Flacourtiaceae, and Rosaceae were the plant families with higher root length colonization, and Polygonaceae recorded the least colonization and was similar with the results of Chanie and Assefa (2013). Root length colonization by AMF for six Acacia species were found from 56 to 80% (Santhaguru and Sadhana 2000). The variation in the extent of root colonization could be related to the different preferences of the AMF to various species (Khade and Rodrigues 2009; David 2008) and mycorrhizal dependencies among plant species. The variations in plant species with relation to the degree of AMF colonization indicated that environmental factors influence the presence or absence of mycorrhizae and their colonization level (Alexander 1989) and such variation occur at genus and family levels (John 1980).

Acokanthera schimpri, Cordia africana, Diospyros abyssinica, Dovyalis abyssinica, Ekeberrgia capensis, Olea europaea, Rhus glutinosa, Rhus retinorrhoea, and Rosa abyssinica plant species had higher hyphal root colonization (Additional file 1: Table S3). Mycorrhizal hyphal colonization were higher in Cordia africana, Diospyros abyssinica, Dovyalis abyssinica, Olea europaea, Rhus glutinosa, Rhus retinorrhoea, and Rosa abyssinica plant species. Mycorrhizal hyphal colonization varied from high to low between different tree species (Chanie and Assefa 2013).

The four AMF structures were identified in all the 32 plants from all the study areas. Anacardiaceae, Boraginaceae, Flacourtiaceae, Meliaceae, Oleaceae, and Rosaceae showed higher hyphal colonization (Additional file 1: Table S4). Similarly, Anacardiaceae, Boraginaceae, Flacourtiaceae, Oleaceae, and Rosaceae showed high mycorrhizal root colonization while high % AC was recorded in Boraginaceae. High % VC was observed in Rosaceae and Meliaceae. Recently, Burni and Hussain (2011) also reported variations in AMF root colonization in various members of Lamiaceae. Different edapho-climatic factors like soil type, nutritional status of soil, soil pH, organic matter, soil moisture, rain fall, temperature, etc. may be responsible for variations in root colonization (Tanvir et al. 2011).

AMF spore size

High spore density was recovered for the spore groups with 50 μm diameter followed by 100 and 300 μm (Table 8). Acaulospora had higher spore density in 50 and 100 μm diameter followed by Glomus while it was the most dominant in the 300 μm diameter group. Gigaspora, Scutellospora, and Entrophospora were the third, fourth, and fifth dominants genera in 50 and 100 μm, respectively. Sclerocystis was the least dominant found only in 100 and 300 μm. Brundrett et al. (1996) reported AMF produce large asexual spores (20–1000+ μm diameter). The fact that Acaulospora and Glomus are dominant genera in high lands of Tigray is related to their sporogenous characteristics. It has been found that Acaulospora and Glomus species usually produce more spores than Gigaspora and Scutellospora species in the same environment (Bever et al. 1996).
Table 8

Spore density (100 g−1 of dry soil) and types of genera at different spore size (μm) categories

Spore size (μm)

Gigaspora

Scutellospora

Glomus

Sclerocystis

Acaulospora

Entrophospora

Sum

50 μm

87.8 ± 2.1a

45.2 ± 1.2a

263.2 ± 5.3a

0.000 ± 0.003a

626.1 ± 11.9a

10.9 ± 0.4a

1784.2 ± 32.0a

100 μm

48.2 ± 2.1b

24.2 ± 1.2b

270.4 ± 5.3a

0.007 ± 0.003a

389.1 ± 11.9b

3.5 ± 0.4b

1079.1 ± 32.0b

300 μm

8.8 ± 2.1c

4.1 ± 1.2c

129.9 ± 5.3b

0.002 ± 0.003a

109.4 ± 11.9c

1.8 ± 0.4c

254.2 ± 32.0c

Means in the same column followed by same letter do not differ significantly at P < 0.05, mean ± SEM

Soil nutrients availability with increasing age of exclosures and slope position

Soil chemical properties of exclosure soil were significantly higher than grazing land (Table 9). This result is supported by Mekuria (2010). Grazing impacts on soil properties depends on grazing intensity, with moderate grazing of 33 years compared with an ungrazed control resulted in higher pH, available P, and Mg (Ajorlo et al. 2011) and available P, total N, Ca, Mg, and K decreased after 1.5 years of heavy grazing compared with an ungrazed control in a tropical pasture. Soil chemical properties at older age exclosure had better pH, EC, P, N, K, OC, and OM than the three young age groups of exclosures. Availability of soil nutrients decreased with decreasing age of exclosure. Foot slope had better soil chemical properties than the middle and upper slope (Table 9). EC showed significantly higher in foot slope followed by middle and upper slope respectively which is in agreement with Mekuria (2010).
Table 9

Soil properties in exclosures and adjacent grazing lands with increasing age of exclosures and slope position

 

pH

EC (msm)

P (ppm)

K (ppm)

N (%)

OC (%)

OM (%)

Land uses

Exclosure

7.7 ± 0.03a

15.8 ± 0.38a

9.1 ± 0.45a

8.8 ± 0.2a

0.5 ± 0.01a

1.7 ± 0.05a

2.9 ± 0.09a

Grazing land

7.2 ± 0.03b

9.4 ± 0.38b

2.9 ± 0.45b

3.7 ± 0.29b

0.2 ± 0.01b

0.3 ± 0.05b

0.5 ± 0.09b

Age groups

< 10

7.2 ± 0.05c

10.4 ± 0.64b

2.2 ± 0.67c

3.85 ± 0.49c

0.3 ± 0.02b

0.4 ± 0.11c

0.7 ± 0.19c

10–20

7.4 ± 0.05b

11.9 ± 0.64b

5.7 ± 0.67b

6.1 ± 0.49b

0.4 ± 0.02b

0.95 ± 0.11b

1.6 ± 0.19b

20–30

7.6 ± 0.05ab

12.4 ± 0.64b

6.16 ± 0.67b

6.7 ± 0.49ab

0.4 ± 0.02ab

1.2 ± 0.11ab

2.1 ± 0.19ab

> 30

7.7 ± 0.05a

15.8 ± 0.64a

9.9 ± 0.67a

8.3 ± 0.50a

0.5 ± 0.02a

1.4 ± 0.11a

2.5 ± 0.19a

Slope position

Foot

7.7 ± 0.04a

14.2 ± 0.58a

7.2 ± 0.66a

6.6 ± 0.47a

0.4 ± 0.02a

1.2 ± 0.10a

2.1 ± 0.18a

Middle

7.5 ± 0.04b

12.4 ± 0.58ab

5.7 ± 0.66a

6.3 ± 0.4a

0.4 ± 0.02b

1.0 ± 0.10ab

1.7 ± 0.18ab

Upper

7.3 ± 0.04b

11.2 ± 0.58c

5.1 ± 0.66a

5.7 ± 0.46a

0.3 ± 0.020b

0.8 ± 0.10b

1.4 ± 0.18b

Means in the same column followed by the same letter do not differ significantly at P < 0.05, mean ± SEM

Correlation between AMF and availability of soil nutrients

Spore density had positively correlated with the soil parameters (Table 10) which is in agreement with the studies of Minal and Anil (2012) found a significant positive correlation with pH (r = 0.85, P < 0.01) and organic carbon (r = 0.68, P < 0.05). Similarly, Arpita et al. (2012) found root colonization were positively correlated with % OC, pH, available nitrogen, and available phosphorus but negatively correlated with available potassium. Spore densities were positively correlated with all soil parameters except soil pH. Don-Rodrigue et al. (2013) and Amal et al. (2013) also found positive correlation of soil properties with AMF.
Table 10

Pearson correlation between soil parameters and AMF spore density (100 g−1 of dry soil) and colonization (%)

AMF parameters

Soil parameters

EC (msm)

P (ppm)

K (ppm)

N (%)

OC (%)

OM (%)

Spore density

0.888*

0.822*

0.897*

0.860*

0.904*

0.904*

HC

0.630*

0.638*

0.720*

0.720*

0.737*

0.737*

MHC

0.678*

0.708*

0.758*

0.731*

0.784*

0.784*

AC

0.599*

0.653*

0.673*

0.647*

0.708*

0.708*

VC

0.628*

0.692*

0.685*

0.645*

0.716*

0.716*

HC hyphal colonization, MHC mycorrhizal hyphal colonization, AC arbuscular colonization, VC vesicular colonization

*Correlation is significant at the 0.05 level

Conclusions

AMF spore density and root colonization were higher in exclosures as compared to grazing land and increased with age of exclosures. Foot slope had greater spore density and root colonization than mid and upper slopes. Soil chemical properties showed significant difference and were highest in the exclosure, oldest age of exclosure and foot slope followed by grazing land, youngest age of exclosure and upper slope. AMF spore density and root colonization were significantly positively correlated to soil chemical properties.

Declarations

Acknowledgements

We are grateful to the three anonymous referees for constructive comments on an earlier version of this manuscript. We want to thank the departments of LaRMEP (Forestry, Physical and Chemical labs), chemistry (Organic, Analytical and Physical labs), and biology (Aquatic ecology and Micro-biology labs) for their permission to do laboratory analysis in their respective laboratories.

Funding

The data collection of the study was supported by the Swedish International Development Authority (SIDA) under the Agroecology and Sustainable development project in Mekelle University. The write-up was funded by the steps towards sustainable forest management with the local communities in Tigray, Northern Ethiopia (ETH 13/0018) project funded by NORAD under the NORHED programme.

Authors’ contributions

EB and KMG generated the idea, designed the study, carried out the experiment works, conducted the sample collection and laboratory analysis, collected and analyzed the data, and wrote the manuscript. TD participated in its design and coordination and helped to draft the manuscript. MH and NS provided the statistical assistance and revised the manuscript draft. All authors read and approved the final manuscript.

Competing interests

The authors declare that they have no competing interests.

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Authors’ Affiliations

(1)
Department of Land Resources Management and Environmental protection, Mekelle University
(2)
Faculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life Sciences
(3)
Department of Natural Resource Economics and Management, Mekelle University

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