Environmental variables vis-a-vis distribution of herbaceous tracheophytes on northern sub-slopes in Western Himalayan ecotone

Abstract

Background

In the northern hemisphere, the north face of the mountains has a high diversity of species which is attributed to the moist and shady conditions at the north face. Other environmental variables may also influence the species diversity on the northern faces of the mountain and therefore needs to be studied in detail. The northern slopes represent three different sub-slopes—north, north east, and north west. During the current investigation of Pir-Panjal and Dauladhar ranges in Bhadarwah valley, fine-scale studies on the relationship between vegetation and four variables (soil pH, moisture content, electrical conductivity, and steepness) were conducted. The study determined the role of these variables on the vegetation of three different sub-slopes. The sampling was done at the confluence of two communities (forest and grassland) at three sites on the northern sub-slopes of the lesser stratum in western Himalayan.

Results

The result revealed that rich herbaceous diversity prevails on the lesser Himalayan stratum (Bhadarwah valley). As many as 65.8% species differ from site to site, whereas species commonality among the sites is minimum. The role of environmental variables on the species composition at different sub-slopes of the north mountain face is deduced through canonical correspondence analysis (CCA).

Conclusions

The availability of solar light increases air and soil temperature on the north east–facing slope. All the environmental variables (soil pH, moisture, electrical conductivity, and mountain steepness) are insignificant at pure north face for the species composition. Therefore, it can be concluded that some other environmental variables may influence the species composition which are needed to be further investigated.

Introduction

The particular mountain face composes a unique habitat for a specific type of plant community (Nowak et al. 2014). Each mountain face shows contrasting characteristics with respect to insolation, light intensity, soil moisture, pH of the soil, humidity, etc. (Pook and Moore 1966; Gupta and Arora 2017). These facets are also closely associated with the vegetation composition and community structure (Gilliam et al. 2014; Kumar et al. 2018). The north-facing slopes retain moisture and are more cold and humid than the south-facing slopes; consequently, they support moisture-loving plants (Erdos et al. 2012). The north slopes offer a better habitat for plant growth and help in adequate regeneration of species (Maren et al. 2015). The north-facing slopes are characterized by higher species diversity than the south-facing slope (Gong et al. 2008; Ucles et al. 2015). These slopes are efficient ecosystems for maintaining a specific microhabitat (Mehrabi et al. 2014). The microhabitat parameters (soil pH, moisture, electrical conductivity, etc.) influence the species richness/heterogeneity on the north-facing slopes (Härdtle et al. 2003; Maren et al. 2015).

Ecosystem being a cybernetic system maintains a specific species composition and richness using various environmental variables (Odland and Birks 1999). Among various environmental variables, altitudinal and latitudinal gradients also contribute to the maintenance of species diversity (Murray 1997; Bhattarai 2017). In this regard, the altitudinal gradients along with geographical coordinates are evaluated for their pivotal role in the distribution of vegetation (Juarez et al. 2007; Kazakis et al. 2007). In recent years, new approaches to quantify changes in species diversity across environmental gradients and boundary regions are also developed (Wilson and Shmida 1984; Koleff et al. 2003; Mena and Domı’nguez 2005). Beta-diversity or species turnover is recognized as a simple modus operandi for comparing the diversity of two landscapes or communities or ecosystems (Wilson and Shmida 1984).

Transition zone also called ecotone has become a central topic in landscape ecology and nature conservation because of high floral and faunal diversity. Therefore, the study of ecotone is one of the most rampant research fields nowadays (Cadenass et al. 2003; Ries et al. 2004; Yarrow and Marin 2007, Erdos et al. 2014). Apart from maximum biodiversity, transition zones are differentiated from their nearby communities because these zones are known for maximum transfer of materials, energy, genotypes, and other environmental variables (Austin 2005; Kumar et al. 2017; Guerin et al. 2019). Ecotone delimits the spatial heterogeneity of a landscape and forms an ideal ecosystem (Fagan et al. 2003; Hou and Walz 2016).

Indian Himalayan Region (IHR), including the Tibetan Plateau, is a unique physico-geographical region with an average elevation of 4000 m above msl (Zheng et al. 1981; Ding et al. 2014). The western Himalayan montane and subalpine vegetation on north slopes primarily consist of coniferous species comprising stands of Abies pindrow (Royle ex D. Don) Royle, Pinus wallichiana A. B. Jacks., Picea smithiana (Wall.) Boiss., and Cedrus deodara (Roxb. ex D. Don) G. Don). At few places, individuals of birch (Betula utilis D. Don) also grow in the upper subalpine belt. These individuals of birch finally turn into a narrow band of more or less pure birch stands (Schickhoff et al. 2015). These stands are followed by the grasslands and pastures at high elevation at various mountain faces. Out of the four major mountain faces (north (N), south (S), east (E), and west (W)), north face is not always known for high densities of mesic temperate species and south face predominate by paleotropical xeric biota (Erdos et al. 2012; Nevo 2001). Moreover, the north and east faces of the mountains are evaluated as more productive slopes (Maren et al. 2015). Therefore, the number of studies on vertical transects has been conducted to understand the ecology and species distribution.

Along the elevational gradient (vertical transect), species richness may decrease with the increase in altitudes on the north face (Grytnes 2003). However, such studies on horizontal plane for sub-slopes of the north face like pure N, north east (NE), and north west (NW) are wanted in the science. Moreover, the role of environmental variables on vegetation at specific sub-face of the north is also not fully tacited by the ecologists. During the current study, sampling sites were selected on the transition zone between forest-grassland communities, because of structural composition and functional value of these ecosystems. Data has been collected to understand the role of environmental variables on herbaceous species diversity at various sub-slopes of the north.

Material and methods

Study area

Himalaya, divisible in three zones (a) Outer, (b) Lesser, and (c) Greater Himalaya, is a unique mountain system with respect to climate, rainfall, soil, topography, etc. Lesser Himalaya is a middle stratum extended from eastern to western Himalaya in ca. 2000 km between Shiwalik Range in the south and Great Himalaya in the north. It is composed of many important temperate ranges like Pir-Panjal, Dauladhar, etc. in western Himalaya. Each range possesses gentle northern and steep southern slopes. Northern slopes of Lesser Himalaya are characterized with thick tree flora ranging from boardleaved species to conifers in western Himalaya. In addition, high altitudes also hold a rich herbaceous species diversity forming grassland ecosystems. The study is conducted in Bhadarwah valley falling in Lesser Himalaya which is located in the north western portion of IHR (Fig. 1). The study area lies between an altitudinal range from 821 to 4400 m above msl on geographical coordinates 33.081′ N and 75.332′ E to 32.514′ N and 75.412′ E (Dutt and Kant 2007). This part of the Himalaya holds a variety of vegetation ranging from sub-tropical to alpine. The area is dominated by coniferous forests from 1200 to 3300 m above msl followed by grassland ecosystems which supports more than 194 medicinal plants (Chander 2005). A total of 488 species belonging to 323 genera represent the flora of the study area. Climatic parameters like annual temperature, relative humidity, and other ombrothermic features are responsible for the predominance of family Asteraceae in Bhadarwah (Kumar 1987). Phytoclimate of the area is thero-hemicryptophytic because of many ecological factors at macro-, meso-, and microclimatic level (Kumar 1987; Chander 2005).

Fig. 1

Study area showing coniferous forest and grassland communities in the lesser stratum of NW Himalaya. a Himalaya mountain system. b Sampling sites (red strips) in the lesser stratum of in NW Himalaya. c A study site showing forest and grassland communities along the altitude gradient in the lesser stratum of NW Himalaya. Source: www.davidzurick.com/maps.html. Red color strips in b indicate the location of sampling sites

Arrangement of plots and sampling

To cover all the possible three sub-slopes (NW, NE, and N) of the northern mountain face, 4 sites (1 site in NW, 1 site in NE, 2 sites in N) at different mountain peaks were identified for the study. The details of the sites are presented in Table 1. Each site was identified at the confluence of forest and grassland communities because such transition zone possesses maximum species diversity. Northern face was selected purposely because this face is always earmarked with wet and shady conditions responsible for high species richness. One hundred continuous sampling units of 1 m² were laid down along each long transect (100 units × 4 sites) (Fig. 2). Each transect was arranged from the interior of the forest to the interior of the grassland communities intersecting ecotone in perpendicular (Pandita and Dutt 2016). Semi-random sampling procedure was adopted to select the site considering forest communities at low and grassland communities at high elevation. Semi-random sampling is a modified form of stratified sampling. In the stratified random sampling method, the study site was divided into a number of homogenous units, and then samples in these units are placed randomly. Evidently, in this approach, all variations in vegetation considered as samples are distributed throughout the area. This method combines advantages of both systematic and random sampling (namely accuracy and statistical validity respectively) (Singh et al. 2010). Data on the herbaceous tracheophytes, their individuals, and other environmental variables were recorded in the peak season of vegetation (June to August 2016). The species were collected and identified using various field guides and taxonomic keys published in local floras (Polunin and Stainton 1984; Sharma and Kachroo 1983). The collected specimens were transformed into herbarium sheets according to published guidelines (Dutt and Bedi 2014).

Table 1 Details of study sites in Lesser Himalayan stratum

Fig. 2

Sampling sites location. a Khanitop. b Below Padhri. c Guldanada. d Budh Ro Kut

Environmental variables

Data for 4 environmental variables namely, pH, electrical conductivity (EC), moisture contents of soil (SM), and steepness in the mountain slope (MS) were collected in situ using portable pH meter (EuTech), field conductivity meter (ME Max digital conductivity meter), soil moisture meter (ELICO DM-33), and inclinometer (BOSCH DNM 60 L), respectively. The reason for in situ analysis was longer sampling durations, and in order to maintain the accuracy of parameters the analysis was done under in situ conditions.

Statistical analysis

Data on environmental variables and individuals of plant species were arranged on Microsoft Excel. Correct names of the species were authenticated using a web-based database (www.theplantlist.org) and taken as the biotic parameter. Canonical correspondence analysis (CCA) is a multivariate method to elucidate the relationships between biological assemblages of species and their environmental variables (Hejcmanovā-Nežerková and Hejcman 2006). The CCA was performed using PAST v. 3.21 software.

Results

Characteristics of the study area

During the current study, ecotone between forest and grassland communities on northern slopes between Pir-Panjal and Dauladhar ranges is explored (Table 1). The data collection reveals that ca. 84 herbaceous species exist in such regions in the Lesser Himalayan part of western Himalaya (Additional file 1: Table S1). Out of 84 species encountered during the study, ca. 5.8% species are recorded common among 3/4 sites; however, ca. 65.8% species differ from site to site (Fig. 3). The species-area curve helped in the demarcation of three communities namely forest, ecotone, and grassland along each transect.

Fig. 3

Percentage of commonality among the species of the studied sites. This figure indicated the percentage of variations among the studied sites

Environmental variables

Substratum is the outcome of interactions of various environmental variables. During the current study, pH was found to be slightly acidic to near alkaline, whereas pH, moisture content, EC, and steepness of the mountain in forest soil range from 6.1 to 6.7, 35 to 39%, 0.008 to 0.016 simens/m, and 63 to 82.7 degree of angle, respectively. Similarly, pH, SM, EC, and MS in transition zone range from 6.4 to 6.7, 33 to 35%, 0.18 to 0.135 simens/m, and 60 to 80.2 degree of angle, respectively. In grassland soil, pH, SM, EC, and MS range from 6.9 to 7.5, 15 to 20.5%, 0.036 to 0.071 simens/m, and 18 to 78.6 degree of angle, respectively (Table 2). Although on the northern hemisphere, north-facing slopes are recognized as rich in biodiversity, but this species richness decreases with an increase in the altitudes (Grytnes 2003). In our study, we tried to investigate the relationship between four environmental variables with species diversity at three sub-slopes of the north face. Data analysis reveals that the NE and NW faces are rich in terms of the herbaceous tracheophytes than pure north sub-slopes (Table 1). pH, SM, EC, and MS also differ on each sub-slopes (N, NE, and NW) as well as on each community (forest, ecotone, and grassland) (Table 2).

Table 2 Environmental variables at different sites among three communities

Relationship of environmental variables and biological assemblage

The results of the CCA are presented in Fig. 4, where lines represent the soil properties and small circle represents the tracheophytes. Forest cover is characterized by stands of coniferous species predominated by Cedrus deodara (Roxb. ex D. Don) G. Don trees. Other tree species like Picea smithiana (Wall.) Boiss., Abies pindrow (Royle ex D.Don) Royle, and Pinus wallichiana A.B. Jacks co-dominate the tree vegetation in the study area. Berberis lycium Royle, Viburnum grandiflorum Wall. ex DC., Quercus baloot Griff., Rubus ellipticus Sm., Rosa webbiana Wall., and Prinsepia utilis Royle form the shrub layer in forest as well as at transition zone between forest and grassland. CCA map reveals that the studied environmental variables influence the herbaceous species at NE and NW mountain faces. Herbaceous tracheophytes like Morina longifolia Wall., Bromus patulus var. falconeri Stapf., Anemone tetrasepala Royle are the most significant species at NW face, and important environmental variables contributing maximum in setting the ecology at this face are MS and SM. However, Bistorta affinis Greene., Medicago lupulina L., Agropyron repens (L.) P. Beauv., and Cardamine impatiens L. are significant species on NE face. The most significant environmental variables at NE face are identified as EC and pH of the soil. At pure N face, species like Fumaria parviflora L., Gnaphalium hypoleucum DC., and Geranium wallichianum D. Don ex Sweet. are significant species (Fig. 4). CCA being a strong statistical tool helps to comprehend the role of soil pH, SM, soil EC, and MS on the biotic variable (density of herbaceous tracheophytes). CCA reveals that environmental variables like soil EC-pH and MS-SM have high degree of impact on the species composition at NE and NW sub-faces, respectively. CCA at 999 random permutations visualized simultaneously the biotic variables and the environmental variables at different mountain aspects on two axes (Fig. 4). Critical analysis on the CCA map indicates that the EC of the soil has more significant role than the pH of the soil on NE face. However, on the NW face, MS is recognized as a chief environmental variable than SM. Herbaceous tracheophytes are insensitive to any of the studied environmental variables on pure north face in Lesser Himalaya. Among herbaceous tracheophytes, few species are more significant on their specific site of occurrence.

Fig. 4

Triplot of the first and second canonical correspondence analysis (CCA) axis of herbaceous tracheophytes environmental variable and their corresponding sampling sites. Full name for abbreviation codes of herbaceous tracheophytes are given in Additional file 1: Table S1. Site codes: KHA, Khanitop; BP, Below Padhri; BRK, Budh Ro Kut; Gul, Guldanada; G, grassland; E, ecotone; F, forest, N, pure north; NW, north west; NE, north east; and KHA-GN, Khanitop grassland north

Discussion

Both north and south slopes of Lesser Himalayan mountain abode coniferous forest at low and grassland communities at high altitudes. Behavior of the mountain’s north face coupled with the environmental variables toward herbaceous tracheophytes has been elucidated by dividing the north slopes into three sub-slopes (pure north, north east, and north west). NE and NW faces are observed as the paramount habitat for the growth of herbaceous tracheophytes. Data collection on the number of species on vertical belt transects from forest to grassland communities intersecting perpendicularly transition zone helped in the identification of 3 communities namely forest, ecotone, and grassland. Presence or absence of individual species indicated that the transition zone is characterized by the maximum number of species and their individuals. Climate, topography, aspect, inclination of slope, soil type, and land use pattern are recognized as the major contributor to vegetation type (Maren et al. 2015). Furthermore, insolation and light intensity also put down a range of microclimates at the multifaceted landscape. These factors are responsible for the diverse type of vegetation at different mountain aspects (Gillingham 2010). Variation in vegetation between two contrasting aspects (mountain faces) is the result of the difference in reception of solar radiation (Ghimire et al. 2010; Paudel and Vetaas 2014). On the northern hemisphere, south-facing slopes receive more sunlight and become more xeric and warmer. Therefore, such slopes support drought-resistant vegetation and are less favorable for tree growth. However, north-facing slopes hold moisture and remain cold as well as humid, thus supporting moisture-loving plants (Nevo 2001; Maren et al. 2015). It has also been implicated that south-facing slopes receive six times higher solar radiation than north-facing slopes. Therefore, the microclimatic conditions on both slopes differ considerably and uphold different levels of biodiversity (Auslander et al. 2003). Ecotones are described as dynamic zones and play several important roles in ecosystem functioning (Pandita and Dutt 2016). Himalayan treeline ecotones show considerable differences in altitudinal position, physiognomy, and species composition (Schickhoff 2005). Detection of ecotones can be done using field data gathered along transects (one dimension) or across a grid (two dimensions). During the current study, data collection on one dimension reveals that the species composition at ecotone varies drastically from site to site. This indicates that beside species interaction other factors also play imperative role in ecotones. Few species act as ecotonal specialists; therefore, the strongest process for species richness in Lesser Himalaya can be additive blending. Additive blending may be because of an intermediate type of microhabitat with respect to pH, moisture content, EC, and steepness of the mountain at ecotone (Table 2).

Out of studied communities, ecotone is a well-studied community because of its unique characteristic features (Erdos et al. 2012). Mohd-Aizat et al. (2014) while working on soils of Ayer Hitam Forest Reserve reported a pH range of 3.12 to 5.28 which is lower compared with the present work. Ashraf et al. (2012) reported a range of pH 5.90 to 6.23, moisture content 13.3 to 19.3%, and EC 207 to 239 μS/cm in grassland soils of Yusmarg Hill Resort. Fauze et al. (2015) in their confirmation on soils of Yamuna Biodiversity Park, Delhi, found that pH varied from 6.82 to 7.90; EC, 96 to 1679 μS/cm; and moisture content, 8.7 to 29.7%, and corroborate with the current findings.

Conclusions

The present work showed that species have very less similarity (ca. 6%) among different sites, indicating that each mountainous habitat is characterized by high species diversity at a coarse scale. EC-pH of the soil on NE-facing slopes and SM-MS are vital variables on NW-facing slopes. The insolation and period of solar energy are also dependent on the position of the slope. The north east slopes receive maximum insolation, since the studied area is placed on the northern hemisphere. The availability of solar light increases air and soil temperature on the north east–facing slope. All the environmental variables (soil pH, moisture, EC, and mountain steepness) are insignificant at pure north face for the species composition. Therefore, it can be concluded that some other environmental variables may influence the species composition which are needed to be further discovered.